Cell-derived decellularized extracellular matrices.

TitleCell-derived decellularized extracellular matrices.
Publication TypeJournal Article
Year of Publication2018
AuthorsHarris, GM, Raitman, I, Schwarzbauer, JE
JournalMethods Cell Biol
Date Published2018
KeywordsAnimals, Biocompatible Materials, Cell Culture Techniques, Cell Differentiation, Cell Movement, Cells, Cultured, Extracellular Matrix, Regeneration, Tissue Engineering, Tissue Scaffolds

<p>The ability to create cell-derived decellularized matrices in a dish gives researchers the opportunity to possess a bioactive, biocompatible material made up of fibrillar proteins and other factors that recapitulates key features of the native structure and composition of in vivo microenvironments. By using cells in a culture system to provide a natural ECM, decellularization allows for a high degree of customization through the introduction of selected proteins and soluble factors. The culture system, culture medium, cell types, and physical environments can be varied to provide specialized ECMs for wide-ranging applications to study cell-ECM signaling, cell migration, cell differentiation, and tissue engineering purposes. This chapter describes a procedure for performing a detergent and high pH-based extraction that leaves the native, cell-assembled ECM intact while removing cellular materials. We address common evaluation methods for assessing the ECM and its composition as well as potential uses for a decellularized ECM.</p>

Alternate JournalMethods Cell Biol
PubMed ID29310794
PubMed Central IDPMC5995326
Grant ListP41 EB001046 / EB / NIBIB NIH HHS / United States
R01 CA160611 / CA / NCI NIH HHS / United States
T32 EB005583 / EB / NIBIB NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States