Cebulantin, a Cryptic Lanthipeptide Antibiotic Uncovered Using Bioactivity-Coupled HiTES. Author Kyuho Moon, Fei Xu, Mohammad Seyedsayamdost Publication Year 2019 Type Journal Article Abstract The majority of natural product biosynthetic gene clusters in bacteria are silent under standard laboratory growth conditions, making it challenging to uncover any antibiotics that they may encode. Herein, bioactivity assays are combined with high-throughput elicitor screening (HiTES) to access cryptic, bioactive metabolites. Application of this strategy in Saccharopolyspora cebuensis, with inhibition of Escherichia coli growth as a read-out, led to the identification of a novel lanthipeptide, cebulantin. Extensive NMR spectroscopic analysis allowed the elucidation of the structure of cebulantin. Subsequent bioactivity assays revealed it to be an antibiotic selective for Gram-negative bacteria, especially against Vibrio species. This approach, referred to as bioactivity-HiTES, has the potential to uncover cryptic metabolites with desired biological activities that are hidden in microbial genomes. Keywords Anti-Bacterial Agents, Humans, High-Throughput Screening Assays Journal Angew Chem Int Ed Engl Volume 58 Issue 18 Pages 5973-5977 Date Published 2019 Apr 23 ISSN Number 1521-3773 DOI 10.1002/anie.201901342 Alternate Journal Angew Chem Int Ed Engl PMCID PMC6779416 PMID 30843641 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML