C. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance.

TitleC. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance.
Publication TypeJournal Article
Year of Publication2020
AuthorsKaletsky, R, Moore, RS, Vrla, GD, Parsons, LR, Gitai, Z, Murphy, CT
Date Published2020 Oct
KeywordsAnimals, Avoidance Learning, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Female, Membrane Proteins, Mutation, Neurons, Pseudomonas aeruginosa, Ribonuclease III, RNA Interference, RNA, Bacterial, RNA, Small Interfering, RNA, Untranslated, Species Specificity, Transforming Growth Factor beta, Wills

<p>Caenorhabditis elegans must distinguish pathogens from nutritious food sources among the many bacteria to which it is exposed in its environment. Here we show that a single exposure to purified small RNAs isolated from pathogenic Pseudomonas aeruginosa (PA14) is sufficient to induce pathogen avoidance in the treated worms and in four subsequent generations of progeny. The RNA interference (RNAi) and PIWI-interacting RNA (piRNA) pathways, the germline and the ASI neuron are all required for avoidance behaviour induced by bacterial small RNAs, and for the transgenerational inheritance of this behaviour. A single P. aeruginosa non-coding RNA, P11, is both necessary and sufficient to convey learned avoidance of PA14, and its C. elegans target, maco-1, is required for avoidance. Our results suggest that this non-coding-RNA-dependent mechanism evolved to survey the microbial environment of the worm, use this information to make appropriate behavioural decisions and pass this information on to its progeny.</p>

Alternate JournalNature
PubMed ID32908307
PubMed Central IDPMC8547118
Grant ListDP1 AI124669 / AI / NIAID NIH HHS / United States
DP1 GM119167 / GM / NIGMS NIH HHS / United States
R01 AG034446 / AG / NIA NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States