Title | Building the Microtubule Cytoskeleton Piece by Piece. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Alfaro-Aco, R, Petry, S |
Journal | J Biol Chem |
Volume | 290 |
Issue | 28 |
Pagination | 17154-62 |
Date Published | 2015 Jul 10 |
ISSN | 1083-351X |
Keywords | Animals, Cytoskeleton, Female, Humans, Microtubule-Associated Proteins, Microtubules, Models, Biological, Molecular Motor Proteins, Spindle Apparatus, Xenopus laevis |
Abstract | <p>The microtubule (MT) cytoskeleton gives cells their shape, organizes the cellular interior, and segregates chromosomes. These functions rely on the precise arrangement of MTs, which is achieved by the coordinated action of MT-associated proteins (MAPs). We highlight the first and most important examples of how different MAP activities are combined in vitro to create an ensemble function that exceeds the simple addition of their individual activities, and how the Xenopus laevis egg extract system has been utilized as a powerful intermediate between cellular and purified systems to uncover the design principles of self-organized MT networks in the cell.</p> |
DOI | 10.1074/jbc.R115.638452 |
Alternate Journal | J Biol Chem |
PubMed ID | 25957410 |
PubMed Central ID | PMC4498055 |
Grant List | DP2 GM123493 / GM / NIGMS NIH HHS / United States R00 GM100013 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States 4R00GM100013 / GM / NIGMS NIH HHS / United States |