A bright future: optogenetics to dissect the spatiotemporal control of cell behavior.

TitleA bright future: optogenetics to dissect the spatiotemporal control of cell behavior.
Publication TypeJournal Article
Year of Publication2019
AuthorsGoglia, AG, Toettcher, JE
JournalCurr Opin Chem Biol
Date Published2019 Feb
KeywordsAnimals, Humans, Optogenetics, Protein Interaction Mapping, Protein Interaction Maps, Proteins, Signal Transduction

<p>Cells sense, process, and respond to extracellular information using signaling networks: collections of proteins that act as precise biochemical sensors. These protein networks are characterized by both complex temporal organization, such as pulses of signaling activity, and by complex spatial organization, where proteins assemble structures at particular locations and times within the cell. Yet despite their ubiquity, studying these spatial and temporal properties has remained challenging because they emerge from the entire protein network rather than a single node, and cannot be easily tuned by drugs or mutations. These challenges are being met by a new generation of optogenetic tools capable of directly controlling the activity of individual signaling nodes over time and the assembly of protein complexes in space. Here, we outline how these recent innovations are being used in conjunction with engineering-influenced experimental design to address longstanding questions in signaling biology.</p>

Alternate JournalCurr Opin Chem Biol
PubMed ID30529586
PubMed Central IDPMC6382565
Grant ListDP2 EB024247 / EB / NIBIB NIH HHS / United States
F30 CA206408 / CA / NCI NIH HHS / United States