|Title||bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Trovisco, V, Belaya, K, Nashchekin, D, Irion, U, Sirinakis, G, Butler, R, Lee, JJ, Gavis, ER, St Johnston, D|
|Date Published||2016 10 28|
bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot(2A2), which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.
|PubMed Central ID||PMC5125753|
|Grant List||R01 GM067758 / GM / NIGMS NIH HHS / United States|