BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila.

TitleBEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila.
Publication TypeJournal Article
Year of Publication2019
AuthorsUeberschär, M, Wang, H, Zhang, C, Kondo, S, Aoki, T, Schedl, P, Lai, EC, Wen, J, Dai, Q
JournalNat Commun
Volume10
Issue1
Pagination5700
Date Published2019 12 13
ISSN2041-1723
KeywordsAnimals, Animals, Genetically Modified, Cell Line, Chromatin, Co-Repressor Proteins, DNA-Binding Proteins, Drosophila melanogaster, Drosophila Proteins, Embryo, Nonmammalian, Mutation, Transcription Factors, Transcriptional Activation
Abstract

<p>The Drosophila genome encodes three BEN-solo proteins including Insensitive (Insv), Elba1 and Elba2 that possess activities in transcriptional repression and chromatin insulation. A fourth protein-Elba3-bridges Elba1 and Elba2 to form an ELBA complex. Here, we report comprehensive investigation of these proteins in Drosophila embryos. We assess common and distinct binding sites for Insv and ELBA and their genetic interdependencies. While Elba1 and Elba2 binding generally requires the ELBA complex, Elba3 can associate with chromatin independently of Elba1 and Elba2. We further demonstrate that ELBA collaborates with other insulators to regulate developmental patterning. Finally, we find that adjacent gene pairs separated by an ELBA bound sequence become less differentially expressed in ELBA mutants. Transgenic reporters confirm the insulating activity of ELBA- and Insv-bound sites. These findings define ELBA and Insv as general insulator proteins in Drosophila and demonstrate the functional importance of insulators to partition transcription units.</p>

DOI10.1038/s41467-019-13558-8
Alternate JournalNat Commun
PubMed ID31836703
PubMed Central IDPMC6911014
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
R01 NS074037 / NS / NINDS NIH HHS / United States
R35 GM126975 / GM / NIGMS NIH HHS / United States
R01 NS083833 / NS / NINDS NIH HHS / United States