Antagonism between and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.

TitleAntagonism between and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.
Publication TypeJournal Article
Year of Publication2021
AuthorsColonnetta, MM, Lym, LR, Wilkins, L, Kappes, G, Castro, EA, Ryder, PV, Schedl, P, Lerit, DA, Deshpande, G
JournalElife
Volume10
Date Published2021 Jan 18
ISSN2050-084X
Abstract

<p>Transcriptional quiescence, an evolutionarily conserved trait, distinguishes the embryonic primordial germ cells (PGCs) from their somatic neighbors. In , PGCs from embryos maternally compromised for () misexpress somatic genes, possibly resulting in PGC loss. Recent studies documented a requirement for Gcl during proteolytic degradation of the terminal patterning determinant, Torso receptor. Here we demonstrate that the somatic determinant of female fate, (), is a biologically relevant transcriptional target of Gcl. Underscoring the significance of transcriptional silencing mediated by Gcl, ectopic expression of a degradation-resistant form of Torso () can activate transcription in PGCs, whereas simultaneous loss of () reinstates the quiescent status of PGCs. Intriguingly, like mutants, embryos derived from mothers expressing in the germline display aberrant spreading of pole plasm RNAs, suggesting that mutual antagonism between Gcl and Torso ensures the controlled release of germ-plasm underlying the germline/soma distinction.</p>

DOI10.7554/eLife.54346
Alternate JournalElife
PubMed ID33459591
PubMed Central IDPMC7843132
Grant List093913 / / Eunice Kennedy Shriver National Institute of Child Health and Human Development /
K22 HL126922 / HL / NHLBI NIH HHS / United States
R21 HD093913 / HD / NICHD NIH HHS / United States
R01 GM138544 / GM / NIGMS NIH HHS / United States
DGE-1656466 / / National Science Foundation /
138544 / GM / NIGMS NIH HHS / United States
R35 GM126975 / GM / NIGMS NIH HHS / United States
126975 / GM / NIGMS NIH HHS / United States
K22HL126922 / HL / NHLBI NIH HHS / United States