Age-related neurodegenerative disease associated pathways identified in retinal and vitreous proteome from human glaucoma eyes.

TitleAge-related neurodegenerative disease associated pathways identified in retinal and vitreous proteome from human glaucoma eyes.
Publication TypeJournal Article
Year of Publication2017
AuthorsMirzaei, M, Gupta, VB, Chick, JM, Greco, TM, Wu, Y, Chitranshi, N, Wall, RVander, Hone, E, Deng, L, Dheer, Y, Abbasi, M, Rezaeian, M, Braidy, N, You, Y, Salekdeh, GHosseini, Haynes, PA, Molloy, MP, Martins, R, Cristea, IM, Gygi, SP, Graham, SL, Gupta, VK
JournalSci Rep
Date Published2017 Oct 04
KeywordsAged, Aging, Alzheimer Disease, Biomarkers, Blood Coagulation, Cholesterol, Complement Activation, Complement System Proteins, Down-Regulation, Electron Transport, Eye Proteins, Female, Glaucoma, Humans, Male, Membrane Transport Proteins, Middle Aged, Mitochondria, Neurodegenerative Diseases, Protein Interaction Maps, Proteome, Quality Control, Retina, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Up-Regulation, Vitreous Body

<p>Glaucoma is a chronic disease that shares many similarities with other neurodegenerative disorders of the central nervous system. This study was designed to evaluate the association between glaucoma and other neurodegenerative disorders by investigating glaucoma-associated protein changes in the retina and vitreous humour. The multiplexed Tandem Mass Tag based proteomics (TMT-MS3) was carried out on retinal tissue and vitreous humour fluid collected from glaucoma patients and age-matched controls followed by functional pathway and protein network interaction analysis. About 5000 proteins were quantified from retinal tissue and vitreous fluid of glaucoma and control eyes. Of the differentially regulated proteins, 122 were found linked with pathophysiology of Alzheimer's disease (AD). Pathway analyses of differentially regulated proteins indicate defects in mitochondrial oxidative phosphorylation machinery. The classical complement pathway associated proteins were activated in the glaucoma samples suggesting an innate inflammatory response. The majority of common differentially regulated proteins in both tissues were members of functional protein networks associated brain changes in AD and other chronic degenerative conditions. Identification of previously reported and novel pathways in glaucoma that overlap with other CNS neurodegenerative disorders promises to provide renewed understanding of the aetiology and pathogenesis of age related neurodegenerative diseases.</p>

Alternate JournalSci Rep
PubMed ID28978942
PubMed Central IDPMC5627288