Age-related neurodegenerative disease associated pathways identified in retinal and vitreous proteome from human glaucoma eyes. Author Mehdi Mirzaei, Veer Gupta, Joel Chick, Todd Greco, Yunqi Wu, Nitin Chitranshi, Roshana Wall, Eugene Hone, Liting Deng, Yogita Dheer, Mojdeh Abbasi, Mahdie Rezaeian, Nady Braidy, Yuyi You, Ghasem Salekdeh, Paul Haynes, Mark Molloy, Ralph Martins, Ileana Cristea, Steven Gygi, Stuart Graham, Vivek Gupta Publication Year 2017 Type Journal Article Abstract Glaucoma is a chronic disease that shares many similarities with other neurodegenerative disorders of the central nervous system. This study was designed to evaluate the association between glaucoma and other neurodegenerative disorders by investigating glaucoma-associated protein changes in the retina and vitreous humour. The multiplexed Tandem Mass Tag based proteomics (TMT-MS3) was carried out on retinal tissue and vitreous humour fluid collected from glaucoma patients and age-matched controls followed by functional pathway and protein network interaction analysis. About 5000 proteins were quantified from retinal tissue and vitreous fluid of glaucoma and control eyes. Of the differentially regulated proteins, 122 were found linked with pathophysiology of Alzheimer's disease (AD). Pathway analyses of differentially regulated proteins indicate defects in mitochondrial oxidative phosphorylation machinery. The classical complement pathway associated proteins were activated in the glaucoma samples suggesting an innate inflammatory response. The majority of common differentially regulated proteins in both tissues were members of functional protein networks associated brain changes in AD and other chronic degenerative conditions. Identification of previously reported and novel pathways in glaucoma that overlap with other CNS neurodegenerative disorders promises to provide renewed understanding of the aetiology and pathogenesis of age related neurodegenerative diseases. Keywords Humans, Membrane Transport Proteins, Down-Regulation, Female, Male, Retina, Tandem Mass Spectrometry, Aging, Proteome, Eye Proteins, Alzheimer Disease, Middle Aged, Mitochondria, Protein Interaction Maps, Spectrometry, Mass, Electrospray Ionization, Aged, Biomarkers, Electron Transport, Up-Regulation, Cholesterol, Neurodegenerative Diseases, Blood Coagulation, Complement Activation, Complement System Proteins, Glaucoma, Quality Control, Vitreous Body Journal Sci Rep Volume 7 Issue 1 Pages 12685 Date Published 2017 Oct 04 ISSN Number 2045-2322 DOI 10.1038/s41598-017-12858-7 Alternate Journal Sci Rep PMCID PMC5627288 PMID 28978942 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML