Acinetobacter baylyi regulates type IV pilus synthesis by employing two extension motors and a motor protein inhibitor.

TitleAcinetobacter baylyi regulates type IV pilus synthesis by employing two extension motors and a motor protein inhibitor.
Publication TypeJournal Article
Year of Publication2021
AuthorsEllison, CK, Dalia, TN, Klancher, CA, Shaevitz, JW, Gitai, Z, Dalia, AB
JournalNat Commun
Volume12
Issue1
Pagination3744
Date Published2021 06 18
ISSN2041-1723
KeywordsAcinetobacter, Biological Transport, Fimbriae Proteins, Fimbriae, Bacterial, Gene Expression Regulation, Bacterial, Molecular Motor Proteins, Virulence
Abstract

Bacteria use extracellular appendages called type IV pili (T4P) for diverse behaviors including DNA uptake, surface sensing, virulence, protein secretion, and twitching motility. Dynamic extension and retraction of T4P is essential for their function, and T4P extension is thought to occur through the action of a single, highly conserved motor, PilB. Here, we develop Acinetobacter baylyi as a model to study T4P by employing a recently developed pilus labeling method. By contrast to previous studies of other bacterial species, we find that T4P synthesis in A. baylyi is dependent not only on PilB but also on an additional, phylogenetically distinct motor, TfpB. Furthermore, we identify a protein (CpiA) that inhibits T4P extension by specifically binding and inhibiting PilB but not TfpB. These results expand our understanding of T4P regulation and highlight how inhibitors might be exploited to disrupt T4P synthesis.

DOI10.1038/s41467-021-24124-6
Alternate JournalNat Commun
PubMed ID34145281
PubMed Central IDPMC8213720
Grant ListDP1 AI124669 / AI / NIAID NIH HHS / United States
R35 GM128674 / GM / NIGMS NIH HHS / United States