Faculty AssistantEllen Brindle-Clark
- Ph.D., Molecular Biology and Genetics, University of California, San Francisco
- B.S., Aerospace Engineering, Massachusetts Institute of Technology
Research AreaGenetics & Genomics
Research FocusSystems and Synthetic Biology of Photosynthetic Organisms
Photosynthesis provides energy for nearly all life on Earth. As humans increasingly change this planet, it is essential that we understand this process and the organisms that perform it. Our lab aims to dramatically accelerate our understanding of photosynthetic organisms by developing and applying novel functional genomics strategies in the green alga Chlamydomonas reinhardtii. In the long run, we dream of engineering photosynthetic organisms to address the challenges that our civilization faces in agriculture, health and energy.
Our lab is focused around two synergistic areas:
I. Systems Biology of Photosynthetic Organisms
Many fundamental systems-level questions about photosynthetic organisms remain unanswered. What is the full set of genes required for photosynthesis? Which parts work together? What do all the uncharacterized parts do?
The green alga Chlamydomonas reinhardtii is a powerful model photosynthetic organism. The green plant photosynthetic apparatus is highly conserved and thus can be studied in Chlamydomonas. Chlamydomonas can grow as a haploid and in the absence of a functional photosynthetic apparatus, allowing rapid isolation of mutants of interest. Its unicellular nature and short doubling time enable higher throughput experiments than alternative systems.
We are developing transformative tools to enable high-throughput studies of gene function in Chlamydomonas. We have developed a new tool, which increases the pace at which mutated genes in Chlamydomonas can be identified by >1,000-fold. We are presently using this tool to develop a genome-wide collection of Chlamydomonas insertion mutants as a powerful resource for the research community.
II. Molecular Mechanisms of Efficient Photosynthesis
Photosynthetic organisms growing in nearly all environments must cope with rapid fluctuations in light intensity. The sunlight intensity in most environments can change dramatically in a fraction of a second due to e.g. clouds or leaves moving in the wind. Yet, almost nothing is known about the molecular mechanisms that enable efficient photosynthesis under fluctuating light. We recently discovered that plants have evolved a mechanism that enhances photosynthetic efficiency in changing light environments. We found that this mechanism works by accelerating fluxes of ions across the photosynthetic (thylakoid) membrane.
The Carbon Concentrating Mechanism (CCM) allows algae to use CO2 much more efficiently than C3 crop plants. If we understood how this CCM works, we could engineer it into crop plants to increase their growth rates and reduce their need for water and fertilizer. We are working with our collaborators in the NSF project Combining Algal and Plant Photosynthesis to identify and transfer CCM components into the model C3 plant Arabidopsis, as a first step towards ultimately enhancing CO2 uptake in wheat and rice. We recently identified a key protein that we think holds the carbon-fixing enzyme Rubisco in the pyrenoid.
A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2019 ;116(37):18445-18454. .
Phase separation in biology and disease-a symposium report. Ann N Y Acad Sci. 2019 ;. .
A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 ;51(4):627-635. .
The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization. Cell. 2017 ;171(1):148-162.e19. .
A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 ;171(1):133-147.e14. .
A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 2016 ;113(21):5958-63. .
An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii. Plant Cell. 2016 ;28(2):367-87. .
Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 ;5:5439. .
Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum. Science. 2009 ;323(5922):1693-7. .
- HHMI-Simons Faculty Scholar, Howard Hughes Medical Institute and Simons Foundation
- National Institutes of Health Director's New Innovator Award, National Institutes of Health
- Air Force Office of Scientific Research Young Investigator Award, Air Force Office of Scientific Research
- Graduate Course Teaching Award, University of California, San Francisco
- NSF Graduate Research Fellowship, National Science Foundation