Martin H. Wühr

Contact
wuhr@princeton.eduResearch Area
Biochemistry, Biophysics & Structural BiologyResearch Focus
Develop and employ quantitative proteomics methods to obtain a systems level understanding of cellular organizationWebsite
How do molecules organize into living systems?
We now have a near complete parts list of all the molecules constituting cells. However, we still only poorly understand how all these tiny molecules self-organize into much larger organelles, cells, and organisms. Our group aims to elucidate principles underlying this organization. Specifically, we study how the proteome partitions between nucleus and cytoplasm. We aim to decipher the underlying molecular mechanisms, and ask how different nuclear composition affects biological function. To address these questions we employ and develop mass-spectrometry based proteomics and combine this technology with computational, biochemical, and imaging approaches. Our main research models are human tissue culture cells and the eggs, cell-free extracts, and embryos of the frog Xenopus laevis.
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The gain-of-function allele bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly. Proc Natl Acad Sci U S A. 2020 ;117(31):18737-18743. .
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Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition. Cell Rep. 2020 ;31(12):107783. .
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Inference of Multisite Phosphorylation Rate Constants and Their Modulation by Pathogenic Mutations. Curr Biol. 2020 ;30(5):877-882.e6. .
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A Click-Chemistry-Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry. Chembiochem. 2020 ;21(1-2):103-107. .
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Bayesian Confidence Intervals for Multiplexed Proteomics Integrate Ion-statistics with Peptide Quantification Concordance. Mol Cell Proteomics. 2019 ;18(10):2108-2120. .
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A Review on Quantitative Multiplexed Proteomics. Chembiochem. 2019 ;20(10):1210-1224. .
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Proteomics of nucleocytoplasmic partitioning. Curr Opin Chem Biol. 2019 ;48:55-63. .
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Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos. Methods Mol Biol. 2019 ;1920:17-32. .
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Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster. Anal Chem. 2018 ;90(8):5032-5039. .
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A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry. Angew Chem Int Ed Engl. 2018 ;57(11):2958-2962. .
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Quantitative Proteomics for Xenopus Embryos II, Data Analysis. Methods Mol Biol. 2018 ;1865:195-215. .
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Quantitative Proteomics of Xenopus Embryos I, Sample Preparation. Methods Mol Biol. 2018 ;1865:175-194. .
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Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the egg. Proc Natl Acad Sci U S A. 2017 ;114(50):E10838-E10847. .
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Degradation of the BAF Complex Factor BRD9 by Heterobifunctional Ligands. Angew Chem Int Ed Engl. 2017 ;56(21):5738-5743. .
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Asymmetries in Cell Division, Cell Size, and Furrowing in the Xenopus laevis Embryo. Results Probl Cell Differ. 2017 ;61:243-260. .
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Vertebrate Embryonic Cleavage Pattern Determination. Adv Exp Med Biol. 2017 ;953:117-171. .
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Generic Theoretical Models to Predict Division Patterns of Cleaving Embryos. Dev Cell. 2016 ;39(6):667-682. .
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The Nuclear Proteome of a Vertebrate. Curr Biol. 2015 ;25(20):2663-71. .
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On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development. Dev Cell. 2015 ;35(3):383-94. .
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MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem. 2014 ;86(14):7150-8. .
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Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database. Curr Biol. 2014 ;24(13):1467-1475. .
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Accurate multiplexed proteomics at the MS2 level using the complement reporter ion cluster. Anal Chem. 2012 ;84(21):9214-21. .
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A model for cleavage plane determination in early amphibian and fish embryos. Curr Biol. 2010 ;20(22):2040-5. .
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Evidence for an upper limit to mitotic spindle length. Curr Biol. 2008 ;18(16):1256-61. .