Ileana M. Cristea

Contact
icristea@princeton.eduFaculty Assistant
Minda AlenaEducation
- Ph.D., Michael Barber Centre for Mass Spectrometry, University of Manchester Institute of Science and Technology
- M.Chem., Medicinal Chemistry, University of Manchester Institute of Science and Technology
Research Area
Microbiology & VirologyResearch Focus
Virology meets Proteomics: Cellular Host Defense versus Virus Immune EvasionThe research of our laboratory is at the interface between virology and proteomics. We are studying cellular mechanisms used in defense against viruses, as well as mechanisms used by viruses to inhibit or hijack host cell processes. For these studies, we use multidisciplinary approaches, integrating molecular virology, microscopy, mass spectrometry-based proteomics, and bioinformatics. We have developed a series of proteomics-based approaches for characterizing cellular processes occurring during the progression of viral infections, and we are exploring several areas of interest:
- Dynamic regulation of host-virus protein-protein and protein-nucleic acid interactions during the progression of an infection
- Mechanisms of sensing of pathogenic DNA within the nuclei of infected cells
- Initiation and propagation of intrinsic and innate immune responses following infection with nuclear-replicating herpesviruses
- Roles and regulation of human deacetylases (histone deacetylases - HDACs and sirtuins - SIRTs) during viral infections
- Global remodeling of cellular organelles during the progression of viral infection
- Developing targeted and large-scale proteomic tools for quantifying proteins, identifying protein interactions, determining the specificity of interactions, defining distinct protein complexes, and measuring the relative stability of interactions
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Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa. mBio. 2022 ;13(5):e0155922. .
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HTT-OMNI: A Web-based Platform for Huntingtin Interaction Exploration and Multi-omics Data Integration. Mol Cell Proteomics. 2022 ;21(10):100275. .
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The 2022 Report on the Human Proteome from the HUPO Human Proteome Project. J Proteome Res. 2022 ;. .
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Nuclear antiviral innate responses at the intersection of DNA sensing and DNA repair. Trends Microbiol. 2022 ;30(11):1056-1071. .
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A TRUSTED targeted mass spectrometry assay for pan-herpesvirus protein detection. Cell Rep. 2022 ;39(6):110810. .
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The Nuclear DNA Sensor IFI16 Indiscriminately Binds to and Diminishes Accessibility of the HSV-1 Genome to Suppress Infection. mSystems. 2022 ;7(3):e0019822. .
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Virus-host protein interactions as footprints of human cytomegalovirus replication. Curr Opin Virol. 2022 ;52:135-147. .
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Want to Publish in ? This Is What You Need to Know!. J Proteome Res. 2022 ;21(12):2837-2839. .
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Restructured membrane contacts rewire organelles for human cytomegalovirus infection. Nat Commun. 2022 ;13(1):4720. .
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Dynamics of huntingtin protein interactions in the striatum identifies candidate modifiers of Huntington disease. Cell Syst. 2022 ;13(4):304-320.e5. .
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CHD4 is recruited by GATA4 and NKX2-5 to repress noncardiac gene programs in the developing heart. Genes Dev. 2022 ;36(7-8):468-482. .
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Lamin post-translational modifications: emerging toggles of nuclear organization and function. Trends Biochem Sci. 2021 ;46(10):832-847. .
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Cardiac proteomics reveals sex chromosome-dependent differences between males and females that arise prior to gonad formation. Dev Cell. 2021 ;56(21):3019-3034.e7. .
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TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis. Nat Cell Biol. 2021 ;23(3):257-267. .
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Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair. Nucleic Acids Res. 2021 ;49(4):2044-2064. .
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Progress Identifying and Analyzing the Human Proteome: 2021 Metrics from the HUPO Human Proteome Project. J Proteome Res. 2021 ;20(12):5227-5240. .
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The human cytomegalovirus protein pUL13 targets mitochondrial cristae architecture to increase cellular respiration during infection. Proc Natl Acad Sci U S A. 2021 ;118(32). .
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The antiviral sirtuin 3 bridges protein acetylation to mitochondrial integrity and metabolism during human cytomegalovirus infection. PLoS Pathog. 2021 ;17(4):e1009506. .
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Subcellular proteomics. Nat Rev Methods Primers. 2021 ;1. .
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Host Innate Immune Response and Viral Immune Evasion During Alphaherpesvirus Infection. Curr Issues Mol Biol. 2021 ;42:635-686. .
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Post-translational modification control of viral DNA sensors and innate immune signaling. Adv Virus Res. 2021 ;109:163-199. .
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A high-stringency blueprint of the human proteome. Nat Commun. 2020 ;11(1):5301. .
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Interrogating Host Antiviral Environments Driven by Nuclear DNA Sensing: A Multiomic Perspective. Biomolecules. 2020 ;10(12). .
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Proteomic Technologies for Deciphering Local and Global Protein Interactions. Trends Biochem Sci. 2020 ;45(5):454-455. .
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Mechanical Stress Regulates Epithelial Tissue Integrity and Stiffness through the FGFR/Erk2 Signaling Pathway during Embryogenesis. Cell Rep. 2020 ;30(11):3875-3888.e3. .
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Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA. Cell Rep. 2020 ;32(4):107943. .
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TRANSPIRE: A Computational Pipeline to Elucidate Intracellular Protein Movements from Spatial Proteomics Data Sets. J Am Soc Mass Spectrom. 2020 ;31(7):1422-1439. .
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The DNA Sensor cGAS is Decorated by Acetylation and Phosphorylation Modifications in the Context of Immune Signaling. Mol Cell Proteomics. 2020 ;19(7):1193-1208. .
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Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection. Nat Commun. 2020 ;11(1):806. .
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RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters. PLoS Pathog. 2020 ;16(2):e1008269. .
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Research on the Human Proteome Reaches a Major Milestone: >90% of Predicted Human Proteins Now Credibly Detected, According to the HUPO Human Proteome Project. J Proteome Res. 2020 ;19(12):4735-4746. .
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The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation. PLoS Pathog. 2020 ;16(12):e1008861. .
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Conservation and divergence of protein pathways in the vertebrate heart. PLoS Biol. 2019 ;17(9):e3000437. .
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Peroxisome Plasticity at the Virus-Host Interface. Trends Microbiol. 2019 ;27(11):906-914. .
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Early-Career Scientists Shaping the World. mSystems. 2019 ;4(3). .
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Mitochondrial Function, Metabolic Regulation, and Human Disease Viewed through the Prism of Sirtuin 4 (SIRT4) Functions. J Proteome Res. 2019 ;18(5):1929-1938. .
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Mechanical Force Induces Phosphorylation-Mediated Signaling that Underlies Tissue Response and Robustness in Xenopus Embryos. Cell Syst. 2019 ;8(3):226-241.e7. .
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Protein interactions and consensus clustering analysis uncover insights into herpesvirus virion structure and function relationships. PLoS Biol. 2019 ;17(6):e3000316. .
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Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. Cancer Cell. 2019 ;35(1):64-80.e7. .
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YfmK is an N-lysine acetyltransferase that directly acetylates the histone-like protein HBsu in . Proc Natl Acad Sci U S A. 2019 ;116(9):3752-3757. .
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Editorial overview: Untangling proteome organization in space and time. Curr Opin Chem Biol. 2019 ;48:A1-A4. .
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Location is everything: protein translocations as a viral infection strategy. Curr Opin Chem Biol. 2019 ;48:34-43. .
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Hdac4 Interactions in Huntington's Disease Viewed Through the Prism of Multiomics. Mol Cell Proteomics. 2019 ;18(8 suppl 1):S92-S113. .
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Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry. Methods Mol Biol. 2019 ;1977:115-143. .
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Contribution of Mass Spectrometry-Based Proteomics to Discoveries in Developmental Biology. Adv Exp Med Biol. 2019 ;1140:143-154. .
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Methods for characterizing protein acetylation during viral infection. Methods Enzymol. 2019 ;626:587-620. .
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Workflows and considerations for investigating protein interactions of viral DNA sensors. Methods Enzymol. 2019 ;625:309-338. .
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Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication. Cell Host Microbe. 2018 ;24(4):526-541.e7. .
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Orchestration of protein acetylation as a toggle for cellular defense and virus replication. Nat Commun. 2018 ;9(1):4967. .
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Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep. 2018 ;22(7):1913-1922. .
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Protein lipoylation: an evolutionarily conserved metabolic regulator of health and disease. Curr Opin Chem Biol. 2018 ;42:76-85. .
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Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS. Cell Syst. 2018 ;7(6):627-642.e6. .
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Efficient inference for time-varying behavior during learning. Adv Neural Inf Process Syst. 2018 ;31:5695-5705. .
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Orchestration of protein acetylation as a toggle for cellular defense and virus replication. Nat Commun. 2018 ;9(1):4967. .
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Exploring and Exploiting Proteome Organization during Viral Infection. J Virol. 2017 ;91(18). .
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Formation of a TBX20-CASZ1 protein complex is protective against dilated cardiomyopathy and critical for cardiac homeostasis. PLoS Genet. 2017 ;13(9):e1007011. .
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Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly. Matrix Biol. 2017 ;59:39-53. .
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Proteomics and integrative omic approaches for understanding host-pathogen interactions and infectious diseases. Mol Syst Biol. 2017 ;13(3):922. .
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Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor. Cell Host Microbe. 2017 ;21(6):731-741.e10. .
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Regulation, Function, and Detection of Protein Acetylation in Bacteria. J Bacteriol. 2017 ;199(16). .
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Transcriptional Elongation of HSV Immediate Early Genes by the Super Elongation Complex Drives Lytic Infection and Reactivation from Latency. Cell Host Microbe. 2017 ;21(4):507-517.e5. .
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Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation. Mol Cell Proteomics. 2017 ;16(4 suppl 1):S200-S214. .
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Proteomics Tracing the Footsteps of Infectious Disease. Mol Cell Proteomics. 2017 ;16(4 suppl 1):S5-S14. .
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The Host-Pathogen Ecosystem Viewed Through the Prism of Proteomics. Mol Cell Proteomics. 2017 ;16(4 suppl 1):S1-S4. .
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HVint: A Strategy for Identifying Novel Protein-Protein Interactions in Herpes Simplex Virus Type 1. Mol Cell Proteomics. 2016 ;15(9):2939-53. .
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Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity. PLoS Genet. 2016 ;12(9):e1006238. .
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A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection. Cell Syst. 2016 ;3(4):361-373.e6. .
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Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking. Mol Cell Proteomics. 2016 ;15(10):3107-3125. .
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Diverse mechanisms evolved by DNA viruses to inhibit early host defenses. Crit Rev Biochem Mol Biol. 2016 ;51(6):452-481. .
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The Proteomic Profile of Deleted in Breast Cancer 1 (DBC1) Interactions Points to a Multifaceted Regulation of Gene Expression. Mol Cell Proteomics. 2016 ;15(3):791-809. .
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HIV-host interactome revealed directly from infected cells. Nat Microbiol. 2016 ;1(7):16068. .
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Intricate Roles of Mammalian Sirtuins in Defense against Viral Pathogens. J Virol. 2016 ;90(1):5-8. .
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The Biochemical Evolution of Protein Complexes. Trends Biochem Sci. 2016 ;41(1):4-6. .
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The Cardiac TBX5 Interactome Reveals a Chromatin Remodeling Network Essential for Cardiac Septation. Dev Cell. 2016 ;36(3):262-75. .
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Two Modes of the Axonal Interferon Response Limit Alphaherpesvirus Neuroinvasion. mBio. 2016 ;7(1):e02145-15. .
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Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements. PLoS Negl Trop Dis. 2016 ;10(8):e0004921. .
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Diverse mechanisms evolved by DNA viruses to inhibit early host defenses. Crit Rev Biochem Mol Biol. 2016 ;:1-30. .
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Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators. Methods Mol Biol. 2016 ;1436:213-39. .
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Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5). Methods Mol Biol. 2016 ;1436:47-84. .
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Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy. Methods Mol Biol. 2016 ;1410:39-63. .
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Proteomic approaches to uncovering virus-host protein interactions during the progression of viral infection. Expert Rev Proteomics. 2016 ;13(3):325-40. .
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Proteomics of yeast telomerase identified Cdc48-Npl4-Ufd1 and Ufd4 as regulators of Est1 and telomere length. Nat Commun. 2015 ;6:8290. .
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Blowing Off Steam: Virus Inhibition of cGAS DNA Sensing. Cell Host Microbe. 2015 ;18(3):270-2. .
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Interactions of the Antiviral Factor Interferon Gamma-Inducible Protein 16 (IFI16) Mediate Immune Signaling and Herpes Simplex Virus-1 Immunosuppression. Mol Cell Proteomics. 2015 ;14(9):2341-56. .
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DNA methyltransferase DNMT3A associates with viral proteins and impacts HSV-1 infection. Proteomics. 2015 ;15(12):1968-82. .
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The functional interactome of PYHIN immune regulators reveals IFIX is a sensor of viral DNA. Mol Syst Biol. 2015 ;11(1):787. .
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ComGA-RelA interaction and persistence in the Bacillus subtilis K-state. Mol Microbiol. 2015 ;97(3):454-71. .
Dr. Ileana Cristea has performed her graduate research at the Michael Barber Center for Mass Spectrometry, University of Manchester, U.K., under the supervision of Simon Gaskell, and at the Toxicology Research & Development Department at GlaxoSmithKline, U.K. She pursued her postdoctoral work in the mass spectrometry laboratory of Brian Chait at The Rockefeller University. She is currently an Associate Professor in the Department of Molecular Biology at Princeton University. The research of her group is at the interface between proteomics and virology. Her goal is to build an understanding of viral infection from a proteomics perspective. Broad questions that her lab is addressing are: How do viruses effectively modulate cellular pathways? How do hosts respond to viral infection? Can proteomics identify key host proteins to harness for therapeutic development? To accomplish these goals, her laboratory utilizes a multi-disciplinary approach that integrates proteomics techniques with genomics, microscopy, bioinformatics, and virology. These approaches have allowed her group to bridge developments in mass spectrometry to critical findings in biology, identifying mechanisms used by viruses to manipulate their hosts, as well as defenses hosts deploy to protect themselves from viral attack. Dr. Cristea is a member of the Executive Board of the US Human Proteome Organization (2012-present), US-HUPO Education Committee (2011-present), the American Society for Mass Spectrometry Education Committee (2008-2010), and on the Editorial Boards of Molecular & Cellular Proteomics, Proteomics,Journal of Proteome Research, andJournal of Neuroimmune Pharmacology. She also chairs the Infectious Disease initiative of the HUPO World organization. She acts as a head instructor of the summer Proteomics Course at Cold Spring Harbor Laboratory, having taught there since 2006. She has taught related workshops at ASMS, HUPO World Congress, US-HUPO, AOHUPO, and ABRF. She is the recipient of the Bordoli Prize from the British Mass Spectrometry Society (2001), NIDA Avant-Garde Director Pioneer Award for HIV/AIDS Research (2008), Human Frontiers Science Program Young Investigator Award (2009), Early Career Award in Mass Spectrometry from the American Chemical Society NJ Section (2011), the American Society for Mass Spectrometry Research Award (2012), and the Molecular & Cellular Proteomics Lectureship (2013).
2015
- Mallinckrodt Scholar Award, Edward Mallinckrodt, Jr. Foundation
2012
- American Society for Mass Spectrometry Research Award, American Society for Mass Spectrometry
2011
- Early Career Award in Mass Spectometry, American Chemical Society