Shelby Blythe (Princeton U)

Shelby Blythe (Princeton U)

Special Seminar

Event Date/Location

February 3, 2016 - 12:00 pm
Thomas Laboratory 003

Speaker

  • Photo of Shelby Blythe

    Shelby Blythe

    Deapartment of Molecular Biology
    Princeton

Topic

Transcription, chromatin remodeling, and cell cycle control during early embryogenesis

Historically, our knowledge of the genetic interactions that shape and pattern the embryo has far surpassed our understanding of how chromatin structure regulates gene expression. I have developed technologies for measuring chromatin accessibility (ATAC-seq) and occupancy (ChIP-seq) to address how changes in chromatin structure operate over short (3 to 20 minute) timescales to alter the gene-regulatory landscape in Drosophila embryos. With these approaches, I have measured the large scale remodeling events that establish a chromatin ‘ground state’, and have characterized how spatially restricted patterns of chromatin accessibility arise in response to patterning information. These large scale changes in chromatin structure are functionally linked to simultaneous cell cycle remodeling events. By exploring this functional interaction, I have developed genetic approaches for identifying rate-limiting factors for driving the large scale coordinated remodeling of embryonic promoters. These experiments serve as the basis for the future systematic examination of how fine-scale control of chromatin architecture underlies the embryonic developmental program.

 

Audience

Free and open to the university community and the public

Host

Elizabeth Gavis, Department of Molecular Biology