SOKENDAI (The Graduate University for Advanced Studies)
Visualization, quantification, and manipulation of ERK signaling with fluorescence live-cell imaging
The Ras-ERK MAPK signaling cascade has been highly conserved throughout evolution and plays a key role in many cellular processes, including proliferation, differentiation, survival, and tumorigenesis. Although the Ras-ERK signaling pathway has been extensively studied for decades, it is still unclear how the Ras-ERK signaling controls such diverse cellular processes. To address this issue, we have developed tools to visualize, quantify, and manipulate intracellular signaling with live cell imaging. Here, I will show two topics in our recent studies about (1) collective cell migration driven by the intercellular propagation of ERK activation, and (2) a genetically-encoded optogenetic tool for red/infrared light-mediated manipulation of cell signaling.
Free and open to the university community and the public.