Mol Bio Colloquium

Mol Bio Colloquium

Graduate Student Colloquium

Event Date/Location

September 30, 2022 - 4:00 pm
Thomas Laboratory 003

Speaker

  • Lisset Duran Photo

    Lisset Duran

    Graduate Student
    Avalos & Cohen Labs

    Lisset’s research involves understanding the molecular mechanisms that lead to resistance in different extremophiles.

     

    She received her B.S from John Jay College at CUNY in molecular biology. There she studied DNA Methylation in Breast Cancer. She has also done summer internships at Brown University studying Acute Lung Injury and at National Institute of Medicinal and Aromatic Plants at Morocco studying the medicinal property of plants.

     

    On her free time, she enjoys painting and taking videos of waterbears.

  • Seraya Jones Nelson

    Graduate Student
    Jimah Lab
  • Caitlin Lamb Photo

    Caitlin Lamb

    Graduate Student
    Myhrvold and te Velthuis Labs

    Prior to coming to Princeton, Caitlin earned her bachelors in chemistry from Duke University and worked with Prof. Peter Klopfer to discover the factors linked to hibernation in the fat-tailed dwarf lemurs and with Prof. Michael Boyce to explore how Coat Protein Complex II is regulated.Here, her project focuses on using Cas13-based mvRNA detection to learn about the dynamics of influenza and other viral infections.

Topic

Graduate Colloquium

CAITLIN LAMB

Myhrvold and te Velthuis Labs

 

Multiplexed detection of influenza A virus mini viral RNAs using Cas13

 

Influenza A virus (IAV) can cause mild to severe respiratory illness in humans, affecting 9 million to 41 million people annually in the United States alone. During infection, IAV produces mini viral RNAs (mvRNAs) that trigger the innate immune response. The extent of the immune response plays a role in disease outcome. The timing and the degree of mvRNA production, along with how those factors influence the innate immune response, remains elusive. Furthermore, there are no sensitive, cost-effective, and specific methods to understand mvRNAs. Thus, I developed a Cas13-based detection assay to quantify mvRNAs in transfected and infected cells. I aim to use this method to study the temporal dynamics of mvRNA production and discover how the production of mvRNAs correlates with the innate immune response in tissue culture, animal models, and clinical samples. Additionally, I will create a multiplexed Cas13-based detection assay to evaluate different species of mvRNAs present in a single sample to gain a better insight into the production of various mvRNAs and disease outcomes. Together, these experiments will allow us to understand the interactions between mvRNAs and innate immunity and help us create models that will revolutionize viral diagnostics.      

 

 

 

 

LISSET DURAN

Avalos and Cohen Labs

 

A novel Yeast-Liquid-Hybrid (YLH) high-throughput method to uncover protein condensate constituents 

 

The field of intracellular condensates is an emerging and growing research area. Liquid membranelles organelles have been found to be ubiquitous in cellular biology with functional roles in signaling, transcription, metabolism, etc. However, our ability to study such condensates in a high throughput manner is limited. Therefore, we sought to redesign and repurpose the classical Yeast-Two-Hybrid (Y2H) system into a new Yeast-Liquid-Hybrid (YLH) method to identify and study partitioning of proteins into phase-separated droplets. We have selected FUS1 and Tardigrade Intrinsically Disordered proteins to test and establish the system. The development of this method will allow for the discovery of interaction at a much larger scale than currently possible. 

 

 

SERAYA JONES NELSON

Jimah Lab

 

Structural basis of dynamin regulation by amphiphysin during endocytosis

 

The extra- and intracellular eukaryotic membranes undergo constant membrane remodeling via concerted cycles of fission and fusion events, essential for many biological processes including nutrient uptake, receptor recycling, cell signaling and endocytosis. The proteins responsible for membrane remodeling include evolutionarily conserved guanosine triphosphatases (GTPases) belonging to the dynamin family. Dynamin is regulated by the integral accessory proteins, however, the mechanism, structural and functional basis by which these integral proteins interact with and regulate dynamin remains elusive. Amphiphysin, a Bin/amphiphysin/Rvs (BAR) domain protein mediates the invagination and fission steps of vesicles by sensing or facilitating membrane curvature and stimulating the GTPase activity of dynamin. Amphiphysin has been shown to regulate dynamin’s GTPase activity, a key requirement for fission, through an unknown mechanism. To understand this, I will elucidate the mechanisms by which amphiphysin co-operates with dynamin to promote fission in endocytosis. To do this, I intend to uncover the structure of full length amphiphysin in solution and in lipid tubules, which mimic their endogenous assembly on the neck of budding endocytic vesicles, using Cryogenic Electron Microscopy (cryo-EM). Additionally, I will conduct GTPase assays and fission assays to measure how amphiphysin modulates dynamin’s GTPase activity and measure the progressive appearance of high curvature fission products upon exposure to vesicles. By utilizing both structural and biophysical studies, the findings derived from this work will provide key insight into the spatio-temporal coordination of membrane fission during endocytosis. The insight obtained through this work will be instrumental in understanding amphiphysin’s regulation of dynamin to form endocytic vesicles involved in intracellular trafficking.

 

Audience

Free and open to the university community and the public.

Host

Department of Molecular Biology