Luciano Marraffini (The Rockefeller University)

Luciano Marraffini (The Rockefeller University)

Butler Seminar Series

Event Date/Location

October 25, 2017 - 12:00 pm
Thomas Laboratory 003


  • Picture of Luciano Marraffinni

    Luciano Marraffini

    Associate Professor
    The Rockefeller University


CRISPR-Cas, the adaptive immune system of prokaryotes

Clustered, regularly interspaced, short palindromic repeat (CRISPR) loci and their associated (Cas) proteins provide adaptive immunity against viral and plasmid infection in prokaryotes. The CRISPR-Cas immune response can be divided into two phases. Upon infection, short phage or plasmid sequences known as spacers integrate between CRISPR repeats. This is known as the immunization stage. During the second phase, the targeting phase, spacers are transcribed into small RNA guides that identify the viral or plasmid targets of CRISPR immunity. The CRISPR RNA guides are loaded into Cas nucleases and direct them to complementary sequences in the invading genome. Cleavage of the target genome results in the end of the infection. I will discuss recent work in my lab which shows how these two phases of the CRISPR-Cas immune response are fundamentally linked.


Free and open to the university community and the public.


Bonnie Bassler, Department of Molecular Biology