The facility is currently equipped with the following instrumentation:
Flow Cytometers
BD Biosciences FACSymphony A3 Analyzer w/ HTS
- State of the art five-laser fixed alignment benchtop flow cytometer.
- 355, 405, 488, 561, & 637nm lasers excitation sources.
- Capable of up to 23-color acquisition utilizing fiber optic technology.
- Low-noise VPX electronics for improved fluorescence detection sensitivity.
- Equipped with high-throughput 96 & 384-well automated microsampler.
- Available by appointment with core staff and to trained users 24/7.
BD Biosciences LSRII Multi-Laser Analyzer w/ HTS
- Five-laser fixed alignment benchtop system.
- 355, 405, 488, 561, & 640nm lasers for excitation.
- Capable of up to 14-color acquisition.
- Equipped with high-throughput 96 & 384-well plate automated microsampler.
- Available by appointment with core staff and to trained users 24/7.
Cell Sorters
BD Biosciences FACSAria Fusion
Custom built five-laser, fixed alignment FACS cell sorter
- 355, 405, 488, 561 and 637nm laser excitation sources.
- Capable of 20-parameter (17-color, 3-scatter) acquisition.
- Four nozzle sizes available (70, 85, 100 & 130 micron) to support sorting of a wider range of cell types and sizes.
- Temperature controlled sorting into tubes or plates.
- Index Sorting which allows post-sort results to be precisely tracked back to the phenotypic characteristics of a single cell within each well of the plate.
- Baker Class II biosafety cabinet for safer, sterile sorting of BSL1 & BSL2 level materials.
- Available by appointment with core facility staff.
Sony Biotechnology MA-900
Four-laser, fixed alignment benchtop FACS cell sorter
- 405, 488, 561 and 638nm laser excitation sources.
- Capable of 14-parameter (12-color, 2-scatter) detection.
- Automated setup: has automated startup, sorting chip to laser alignment and drop delay calculation.
- Automated operation monitoring: has droplet breakoff and sample volume monitoring for walk-away sorting.
- Automated fluidics monitoring.
- Housed in a Baker Class II biosafety cabinet for safer, sterile sorting of BSL1 & BSL2 level materials.
- Temperature control of the sort and sample chambers.
- Available by appointment with core facility staff and to trained users 24/7.
Bio-Rad Laboratories S3e Cell Sorter
Dual laser (488 & 561nm) benchtop cell sorter.
- Capable of 6 parameter (4-color, 2-scatter) detection.
- Automated setup: has automated startup, stream-to-laser alignment and drop delay calculation.
- Automated operation monitoring: has droplet breakoff , sample volume, and sort collection tube volume monitoring for walk-away sorting.
- Automated fluidics monitoring: all reservoirs are self-monitoring and display the amount of time before a swap is required.
- Fluids may be live-swapped during a sort.
- Temperature control of the sort and sample tube holders.
- Available by appointment with core facility staff and to trained users 24/7.
COPAS BioSort Worm Sorter
- Large particle cytometer for analyzing, sorting and dispensing cells and organisms 40-175µm in size.
- 488nm laser excitation line with five parameter (3-color) acquisition.
- Updated with a new laser and optics in April 2018.
- ReFlx system for sampling directly from 96-well plates.
- Profiler II option that allows for the spatial mapping of fluorescence signals along the length of the organism.
- Available by appointment with core facility staff and to trained users 24/7.
Particle and Cell Counters
Beckman Coulter Z2 Particle Counter
- 100µm aperture for particle sizing and counting via Coulter Principle (Electrical Sensing Zone Method).
- Useful for yeast and mammalian cell counts.
- Reports count, concentration, and size distribution results.
- Equipped with AccuComp software for improved data analysis and presentation.
- Available to trained users 24/7.
Bio-Rad TC-20 Cell Counter
- Slide-based automated instrument.
- Used for cells 6-50 um in diameter.
- Counts cells per mL of suspension.
- Can count single or mixed population samples.
- Generates a histogram of cell size.
- Can export digital images.
- Quantifies % dead cells using Trypan blue.
- Available to trained users 24/7.
Centrifuge
Cytospin
- Thin-layer cell preparation system.
- Uses centrifugation to deposit cells onto a clearly defined area of a glass slide.
- Can process up to 12 specimens at one time.
- Available to trained users 24/7.
Analytical Software
Site license use of data analysis software platforms from researchers’ personal computers is now available to all Princeton clients. Contact core facility staff for access.
- FCS Express
- FlowJo