Facility Use

The Flow Cytometry Resource Facility provides support for faculty and student research at Princeton University, as well as for investigators outside the university at other academic institutes or private companies. In addition to providing access to the flow cytometers and cell sorting services, we provide technical support to assist in experimental design, data analysis and interpretation as well as development of novel flow cytometric based techniques.

The Flow Cytometry Resource Facility is available 24/7 for use of the flow cytometry benchtop analyzers by fully trained and authorized clients. Training, cell sorting (FACS), and/or consultation appointments can be made by contacting the Facility Director or via the iLab link.

Commonly Used Applications and Fluorochromes

  • Cell phenotyping with fluorescently tagged monoclonal antibodies. Common fluorochromes include: FITC; Alexa350, AL405, AL488, AL568, AL594,  AL633, AL647, AL700, AL750, BB515, PE, PE-Cy5, PE-Cy7, PE-TxRed, PerCP-Cy5.5, APC, APC-Cy7, APC-H7, APC-eFluor780, Pacific Blue, AmCyan, BV421, BV510, BV605, BV650, BV711, V450, V500, eFluor450, BUV395, BUV496, BUV661, and more.

  • Cell cycle analysis and DNA content determination; proliferation; apoptosis/cell death; and cell counting assays: Propidium iodide (PI); Hoechst 33342; DAPI; Sytox Green; SYBR Green; Syto13; Pyronin Y; Acridine Orange; CFDA-SE; Click-It Edu, CellTrace dyes and others.

  • Detection of one or more fluorescent proteins: EGFP, YFP, Venus, Citrine, CyanFP, DsRed, HcRed, mRFP, TagRFP, mCherry, tdTomato, TagBFP, iRFP and others. The new multi-laser systems make it possible to simultaneously detect- and FACS sort-  three or more fluorescent proteins.

  • Functional and biochemical experiments such as RNA levels in individual cells, calcium flux, glutathione levels, phosphorylation states; measurement of intracellular proteins and transcription factors; bead array assays for multiplexed detection of cytokines and other secreted compounds; gain of function and loss of function studies and much, much more!

Please contact the Facility Director to discuss how your research might benefit from flow cytometry or FACS.