Flow Cytometry Resource Facility

The Flow Cytometry Resource Facility provides support for faculty and student research at Princeton University, as well as for investigators outside the university at other academic institutes or private companies. In addition to providing access to the flow cytometers and cell sorting services, we provide technical support to assist in experimental design, data analysis and interpretation as well as development of novel flow cytometric based techniques.

Flow cytometry is a laser based analytical tool in which cells or small organisms in suspension flow at high speed past one or more focused laser light sources. Fluorescently labeled probes and dyes can be added to the sample to determine specific physical and/or biochemical properties of the cells. Optical filters and detectors direct and measure the scattered laser light as well as the fluorescence that is emitted from the cell. Various software platforms are used to acquire and analyze the data, which can be exported in several formats for presentation.

Fluorescence Activated Cell Sorting (FACS) provides all of the same data as flow cytometry and also allows for the sterile recovery of specific populations of interest for further study.

The facility analyzes and sorts a wide range of cell types and species including human, murine, porcine and insect cell lines as well as primary murine leukocytes and stem cells, bacteria, yeast, nuclei, drosophila, zebrafish, aquatic organisms and C. elegans, which reflects the diversity of the research at the University.

Additional services are available at our consortium partner location at the Rutgers Cancer Institute of NJ, Immune Monitoring and Flow Cytometry Shared Resource.

Acknowledgments

We appreciate that you have chosen our facility to conduct your study and would like to remind you to please add a statement along these lines to acknowledge the Flow Cytometry Resource Facility in your publications and presentations:

“We thank Christina DeCoste and/or Katherine Rittenbach and the Molecular Biology Flow Cytometry Resource Facility, which is partially supported by the Rutgers Cancer Institute of New Jersey NCI-CCSG P30CA072720-5921.”

If your data is collected on the FACSymphony flow cytometer, please also acknowledge the funding source from the S10 Shared Instrumentation Grant:  S10OD028592.

NIH Public Access Policy: Publications that result from services provided by this Shared Resource must be compliant with the NIH Public Access policy by submitting your paper to PubMed Central. More information on PubMed Central's methods submission instructions.