Electron Microscopy Core Facility

The Electron Microscopy (EM) Core Facility is administered and supported by the Department of Molecular Biology in collaboration with the University’s Imaging & Analysis Center (IAC), located on the lower level of the Andlinger Center for Energy and the Environment (ACEE).
We provide multiple services encompassing the negative staining, Cryo-EM single-particle analysis (SPA) and tomography (Cryo-ET) pipelines.

  • Technical questions
  • Project design
  • Specimen optimization
  • Data processing


  • Microscopy training (alignments, screening, data collection, troubleshooting)
  • Sample preparation
  • Data processing

Sample preparation equipment is located in Moffett Hall, Room 113. In addition to cryo-EM grid preparation via plunge freezing, we support other sample preparation methods including  immuno-labeling and high-pressure freezing.
The EM Core Facility is open to all Princeton faculty, staff, and students, as well as to off-campus investigators and users.
Those intending to conduct an EM-based project should first to contact the facility manager, Jonathan Bouvette, to discuss specific needs and to design a plan.

Links to useful resources: