Nikon TIRF, Super Resolution (localization type), and Ablation

  • The microscope base is a fully motorized Nikon Ti-E with Perfect Focus System (interferometric-based focus maintenance (PFS)). The system also has a piezo stage with a 100µm range capable of up to 100 steps/sec, mounted in a fully motorized XY stage.
  • TIRF module with mag optic and ¼ wave for super-resolution localization applications.
  • An Agilent high-power MLC400 provides:

         150 mW    647 nm

          70 mW      561 nm

          70 mW      488 nm

          25 mW      405 mW

  • In addition to TIRF capabilities, this system has been optimized for super-resolution capabilities (localization type).
  • System is fitted with Rapp Optoelectronic 355 nm pulsed laser for ablation.
  • There is a 512 back-thinned EMCCD (Andor iXon Ultra) fitted onto this system.
  • A stage piezo also allows for very fast z-section acquisition.
  • There is also an environmental control chamber for long-term live cell imaging.

Best Applications:

Samples less than one hundred microns thick (sample dependent).  Live or fixed (temp. and CO2 available), optimized for live-cell. High magnification, high temporal and spatial resolution imaging.  Ablation and FRAP/PA/PC capabilities. Color tiling.