Structured illumination based super-resolution microscope delivering twice the resolution of traditional diffraction limited microscopes. N-SIM offers temporal resolutions of up to 0.6sec/frame, providing an ideal platform for live-cell imaging at super-resolution. Various imaging modes are available, including TIRF-SIM, 2D-SIM and 3D-SIM. In addition, Princeton’s N-SIM system is equipped with an emission splitter and two cameras enabling simultaneous two color super-resolution imaging.

Best Application/s:

Thinner (less than 10 um) bright samples.  Lower temporal resolution.  100X only.  Standard fluorophores and sample prep.