The microscope base is a fully motorized Nikon Ti2 with Perfect Focus System (PFS, interferometric-based focus maintenance). The system also has a piezo stage with a 200 μm range capable of up to 100 steps/sec, mounted in a fully motorized XY stage. There is also an environmental control chamber for long-term live-cell imaging. Laser Platform A Yokogawa spinning disc (CSU-W1) is mounted with three dichroics: a quad dichroic accommodating 405/488/561/647 nm lasers, a quad dichroic accommodating 405/488/561/690 nm lasers, and a dual dichroic accommodating 454/514 nm lasers. The spinning disc detector is a Hamamatsu BT Fusion sCMOS (6.5 μm pixel with 93% QE). Features a wider field of view and higher image quality than previous models. 2x Resolution Improvement vs. Widefield Enhanced Resolution and Confocal Sectioning Simultaneously Normal Confocal Mode and SoRa Mode Ultra‐wide Field of View Enhanced Signal‐to‐Noise Ratio Flexible Configurations XY Resolution to 120nm Utilizing magnification and microlensing, an approximately 1.4x improvement beyond the optical limit is achieved, which can further be improved by deconvolution to 2x. The field of view of the CSU-W1 is nearly 4 times that of the CSU-X1, making it ideal for large area scanning applications and low magnification imaging of large specimens. This large field of view is a perfect match for large format sensors. CSU-W1 can be configured with a motorized relay lens for magnification switching, allowing users to change magnification to the detector without changing the microscope’s objective lens. Super-resolution images are acquired optically, on any sample, without special preparations or computation. This allows image collection to be limited only by the sample’s signal-to-noise ratio and the exposure time of the detector. Video-rate or faster super-resolution imaging is possible. Microlensing the emission pinhole at the correct magnification counteracts the mismatch between an on-axis excitation PSF and the effective confocal PSF (product of excitation and emission PSFs) through an emission pinhole that is not infinitely small. By microlensing, individual points’ divergence angle onto the pinhole is reduced 2x, mimicking the effect of an infinitely small ideal pinhole, but not compromising signal brightness. Best Applications Samples less than one hundred microns thick (sample dependent). Live or fixed (temperature and CO2 available), optimized for live-cell, high/low magnification, high temporal and spatial resolution, and super-resolution capabilities.
