The MuVi SPIM is a horizontal setup designed to image large volumes very fast. The unique 4-axis concept with its two-sided illumination allows four combinations of illumination and detection.

• The lenses project two aligned light-sheets from opposing directions on the sample. The expandable, alignment-free light source allows a range of four lasers (405, 488, 561, and 642, with 40 mW each before fiber).
• This system is also fitted with the Cleared Sample (CS) chamber, for looking at samples as large as 10 mm x 10 mm x 20 mm.
• The Photo Manipulation module allows you to photobleach fluorescence in selected regions of interest for FRAP or activate proteins for optogenetics

Best Applications:  Typical applications of the microscope include in toto imaging of Zebrafish embryos, Drosophila larvae, marine biology samples, plant and root development, and large cleared tissue samples. Adding the photomanipulation unit provides further flexibility, enabling optical interaction with the samples.