@article{4676,
  author = {Xiao Li and Xiaona Tang and Xinyang Bing and Christopher Catalano and Taibo Li and Gabriel Dolsten and Carl Wu and Michael Levine},
  title = {GAGA-associated factor fosters loop formation in the Drosophila genome.},
  abstract = { <p>The impact of genome organization on the control of gene expression persists as a major challenge in regulatory biology. Most efforts have focused on the role of CTCF-enriched boundary elements and TADs, which enable long-range DNA-DNA associations via loop extrusion processes. However, there is increasing evidence for long-range chromatin loops between promoters and distal enhancers formed through specific DNA sequences, including tethering elements, which bind the GAGA-associated factor (GAF). Previous studies showed that GAF possesses amyloid properties in\&nbsp;vitro, bridging separate DNA molecules. In this study, we investigated whether GAF functions as a looping factor in Drosophila development. We employed Micro-C assays to examine the impact of defined GAF mutants on genome topology. These studies suggest that the N-terminal POZ/BTB oligomerization domain is important for long-range associations of distant GAGA-rich tethering elements, particularly those responsible for promoter-promoter interactions that coordinate the activities of distant paralogous genes.</p> },
  year = {2023},
  journal = {Mol Cell},
  month = {2023/03/28},
  issn = {1097-4164},
  doi = {10.1016/j.molcel.2023.03.011},
  language = {eng},
}