@article{4671,
  author = {Zong-Yen Wu and Wan Sun and Yihui Shen and Jimmy Pratas and Patrick Suthers and Ping-Hung Hsieh and Sudharsan Dwaraknath and Joshua Rabinowitz and Costas Maranas and Zengyi Shao and Yasuo Yoshikuni},
  title = {Metabolic engineering of low-pH-tolerant non-model yeast, , for production of citramalate.},
  abstract = { <p>Methyl methacrylate (MMA) is an important petrochemical with many applications. However, its manufacture has a large environmental footprint. Combined biological and chemical synthesis (semisynthesis) may be a promising alternative to reduce both cost and environmental impact, but strains that can produce the MMA precursor (citramalate) at low pH are required. A non-conventional yeast, , may prove ideal, as it can survive extremely low pH. Here, we demonstrate the engineering of for citramalate production. Using sequence similarity network analysis and subsequent DNA synthesis, we selected a more active citramalate synthase gene () variant for expression in . We then adapted a piggyBac transposon system for that allowed us to simultaneously explore the effects of different gene copy numbers and integration locations. A batch fermentation showed the genome-integrated- strains produced 2.0\&nbsp;g/L citramalate in 48\&nbsp;h and a yield of up to 7\% mol citramalate/mol consumed glucose. These results demonstrate the potential of as a chassis for citramalate production.</p> },
  year = {2023},
  journal = {Metab Eng Commun},
  volume = {16},
  pages = {e00220},
  month = {2023/06/30},
  issn = {2214-0301},
  doi = {10.1016/j.mec.2023.e00220},
  language = {eng},
}