@article{4359,
  keywords = {Animals, Drosophila, Promoter Regions, Genetic, Transcription, Genetic, Gene Expression Regulation, Developmental, Genome, Insect, Single-Cell Analysis, Chromosomes, Insect, Enhancer Elements, Genetic, Regulatory Sequences, Nucleic Acid, Genes, Insect, Chromatin, Genes, Homeobox},
  author = {Philippe Batut and Xin Bing and Zachary Sisco and Jo{\~a}o Raimundo and Michal Levo and Michael Levine},
  title = {Genome organization controls transcriptional dynamics during development.},
  abstract = { <p>Past studies offer contradictory claims for the role of genome organization in the regulation of gene activity. Here, we show through high-resolution chromosome conformation analysis that the  genome is organized by two independent classes of regulatory sequences, tethering elements and insulators. Quantitative live imaging and targeted genome editing demonstrate that this two-tiered organization is critical for the precise temporal dynamics of Hox gene transcription during development. Tethering elements mediate long-range enhancer-promoter interactions and foster fast activation kinetics. Conversely, the boundaries of topologically associating domains (TADs) prevent spurious interactions with enhancers and silencers located in neighboring TADs. These two levels of genome organization operate independently of one another to ensure precision of transcriptional dynamics and the reliability of complex patterning processes.</p> },
  year = {2022},
  journal = {Science},
  volume = {375},
  pages = {566-570},
  month = {2022 Feb 04},
  issn = {1095-9203},
  doi = {10.1126/science.abi7178},
  language = {eng},
}