@article{4244,
  keywords = {Animals, Disease Models, Animal, Humans, Female, Single-Cell Analysis, Virus Replication, Lung, Lymphocyte Activation, Antibodies, Neutralizing, Antibodies, Viral, COVID-19, SARS-CoV-2, COVID-19 Vaccines, Immunization, Secondary, Mesocricetus, 2019-nCoV Vaccine mRNA-1273},
  author = {Michelle Meyer and Yuan Wang and Darin Edwards and Gregory Smith and Aliza Rubenstein and Palaniappan Ramanathan and Chad Mire and Colette Pietzsch and Xi Chen and Yongchao Ge and Wan Cheng and Carole Henry and Angela Woods and LingZhi Ma and Guillaume Stewart-Jones and Kevin Bock and Mahnaz Minai and Bianca Nagata and Sivakumar Periasamy and Pei-Yong Shi and Barney Graham and Ian Moore and Irene Ramos and Olga Troyanskaya and Elena Zaslavsky and Andrea Carfi and Stuart Sealfon and Alexander Bukreyev},
  title = {Attenuated activation of pulmonary immune cells in mRNA-1273-vaccinated hamsters after SARS-CoV-2 infection.},
  abstract = { <p>The mRNA-1273 vaccine is effective against SARS-CoV-2 and was granted emergency use authorization by the FDA. Clinical studies, however, cannot provide the controlled response to infection and complex immunological insight that are only possible with preclinical studies. Hamsters are the only model that reliably exhibits severe SARS-CoV-2 disease similar to that in hospitalized patients, making them pertinent for vaccine evaluation. We demonstrate that prime or prime-boost administration of mRNA-1273 in hamsters elicited robust neutralizing antibodies, ameliorated weight loss, suppressed SARS-CoV-2 replication in the airways, and better protected against disease at the highest prime-boost dose. Unlike in mice and nonhuman primates, low-level virus replication in mRNA-1273-vaccinated hamsters coincided with an anamnestic response. Single-cell RNA sequencing of lung tissue permitted high-resolution analysis that is not possible in vaccinated humans. mRNA-1273 prevented inflammatory cell infiltration and the reduction of lymphocyte proportions, but enabled antiviral responses conducive to lung homeostasis. Surprisingly, infection triggered transcriptome programs in some types of immune cells from vaccinated hamsters that were shared, albeit attenuated, with mock-vaccinated hamsters. Our results support the use of mRNA-1273 in a 2-dose schedule and provide insight into the potential responses within the lungs of vaccinated humans who are exposed to SARS-CoV-2.</p> },
  year = {2021},
  journal = {J Clin Invest},
  volume = {131},
  month = {2021 Oct 15},
  issn = {1558-8238},
  doi = {10.1172/JCI148036},
  language = {eng},
}