@article{3929,
  keywords = {Animals, Mice, Humans, Signal Transduction, Protein Binding, HEK293 Cells, NIH 3T3 Cells, Optogenetics, Protein Transport, Synthetic Biology},
  author = {Agnieszka Gil and C{\'e}sar Carrasco-L{\'o}pez and Liyuan Zhu and Evan Zhao and Pavithran Ravindran and Maxwell Wilson and Alexander Goglia and Jos{\'e} Avalos and Jared Toettcher},
  title = {Optogenetic control of protein binding using light-switchable nanobodies.},
  abstract = { <p>A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few tools confer light-switchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in cells or light-based protein purification in vitro. Here, we report the development of opto-nanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications including reversibly binding to endogenous intracellular targets, modulating signaling pathway activity, and controlling binding to purified protein targets in vitro. This work represents a step towards programmable photoswitchable regulation of a wide variety of target proteins.</p> },
  year = {2020},
  journal = {Nat Commun},
  volume = {11},
  pages = {4044},
  month = {2020 Aug 13},
  issn = {2041-1723},
  doi = {10.1038/s41467-020-17836-8},
  language = {eng},
}