@article{3864,
  keywords = {Transcription Factors, Protein Binding, DNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, RNA Polymerase II, Histones, Nucleosomes, Chromatin, Ubiquitination, Molecular Chaperones, High Mobility Group Proteins, Transcriptional Elongation Factors},
  author = {Magdalena Murawska and Tam{\'a}s Schauer and Atsushi Matsuda and Marcus Wilson and Thomas Pysik and Felix Wojcik and Tom Muir and Yasushi Hiraoka and Tobias Straub and Andreas Ladurner},
  title = {The Chaperone FACT and Histone H2B Ubiquitination Maintain S.~pombe Genome Architecture through Genic and Subtelomeric Functions.},
  abstract = { <p>The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA polymerase II (Pol II) passage through chromatin, yet it is not clear how they cooperate mechanistically. We used genomics, genetic, biochemical, and microscopic approaches to dissect their interplay in Schizosaccharomyces pombe. We show that FACT and H2Bub globally repress antisense transcripts near the 5{\textquoteright} end of genes and inside gene bodies, respectively. The accumulation of these transcripts is accompanied by changes at genic nucleosomes and Pol II redistribution. H2Bub is required for FACT activity in genic regions. In the H2Bub mutant, FACT binding to chromatin is altered and its association with histones is stabilized, which leads to the reduction of genic nucleosomes. Interestingly, FACT depletion globally restores nucleosomes in the H2Bub mutant. Moreover, in the absence of Pob3, the FACT Spt16 subunit controls the 3{\textquoteright} end of genes. Furthermore, FACT maintains nucleosomes in subtelomeric regions, which is crucial for their compaction.</p> },
  year = {2020},
  journal = {Mol Cell},
  volume = {77},
  pages = {501-513.e7},
  month = {2020 Feb 06},
  issn = {1097-4164},
  doi = {10.1016/j.molcel.2019.11.016},
  language = {eng},
}