@article{3814,
  keywords = {Animals, Gene Expression Profiling, Caenorhabditis elegans, Signal Transduction, Mutation, Phenotype, Aging, Caenorhabditis elegans Proteins, Longevity, Receptor, Insulin},
  author = {Rachel Kaletsky and Coleen Murphy},
  title = {Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age.},
  abstract = { <p>Multicellular organisms are composed of distinct cells and tissues that coordinate highly orchestrated responses to environmental challenges, including those that arise with age. Since C. elegans is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe a method for the FACS-based isolation and RNA sequencing of dissociated cells from adult C. elegans. This technique is amenable to profiling the cell- and tissue-specific gene expression changes in C. elegans mutants, including aging models, such as the daf-2/insulin-like signaling (IIS) pathway, and in wild-type animals with age.</p> },
  year = {2020},
  journal = {Methods Mol Biol},
  volume = {2144},
  pages = {177-186},
  month = {2020},
  issn = {1940-6029},
  doi = {10.1007/978-1-0716-0592-9_16},
  language = {eng},
}