@article{3798,
  keywords = {Animals, Drosophila Proteins, Transcription Factors, Cell Line, Mutation, DNA-Binding Proteins, Animals, Genetically Modified, Drosophila melanogaster, Embryo, Nonmammalian, Transcriptional Activation, Chromatin, Co-Repressor Proteins},
  author = {Malin Uebersch{\"a}r and Huazhen Wang and Chun Zhang and Shu Kondo and Tsutomu Aoki and Paul Schedl and Eric Lai and Jiayu Wen and Qi Dai},
  title = {BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila.},
  abstract = { <p>The Drosophila genome encodes three BEN-solo proteins including Insensitive (Insv), Elba1 and Elba2 that possess activities in transcriptional repression and chromatin insulation. A fourth protein-Elba3-bridges Elba1 and Elba2 to form an ELBA complex. Here, we report comprehensive investigation of these proteins in Drosophila embryos. We assess common and distinct binding sites for Insv and ELBA and their genetic interdependencies. While Elba1 and Elba2 binding generally requires the ELBA complex, Elba3 can associate with chromatin independently of Elba1 and Elba2. We further demonstrate that ELBA collaborates with other insulators to regulate developmental patterning. Finally, we find that adjacent gene pairs separated by an ELBA bound sequence become less differentially expressed in ELBA mutants. Transgenic reporters confirm the insulating activity of ELBA- and Insv-bound sites. These findings define ELBA and Insv as general insulator proteins in Drosophila and demonstrate the functional importance of insulators to partition transcription units.</p> },
  year = {2019},
  journal = {Nat Commun},
  volume = {10},
  pages = {5700},
  month = {2019 Dec 13},
  issn = {2041-1723},
  doi = {10.1038/s41467-019-13558-8},
  language = {eng},
}