@article{3725,
  keywords = {Animals, Gene Expression Profiling, Humans, Binding Sites, High-Throughput Screening Assays, RNA, Mass Spectrometry, Proteomics, Proteins},
  author = {Misha Nechay and Ralph Kleiner},
  title = {High-throughput approaches to profile RNA-protein interactions.},
  abstract = { <p>RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.</p> },
  year = {2020},
  journal = {Curr Opin Chem Biol},
  volume = {54},
  pages = {37-44},
  month = {2020 Feb},
  issn = {1879-0402},
  doi = {10.1016/j.cbpa.2019.11.002},
  language = {eng},
}