@article{3704,
  keywords = {Humans, Binding Sites, Protein Binding, Models, Molecular, Protein Conformation, Amino Acid Sequence, Sequence Deletion, Amino Acid Motifs, Cryoelectron Microscopy, Allosteric Regulation, Apoproteins, Calcium Channel Blockers, Piperidines, Calcium Channels, T-Type},
  author = {Yanyu Zhao and Gaoxingyu Huang and Qiurong Wu and Kun Wu and Ruiqi Li and Jianlin Lei and Xiaojing Pan and Nieng Yan},
  title = {Cryo-EM structures of apo and antagonist-bound human Ca3.1.},
  abstract = { <p>Among the ten subtypes of mammalian voltage-gated calcium (Ca) channels, Ca3.1-Ca3.3 constitute the T-type, or the low-voltage-activated, subfamily, the abnormal activities of which are associated with epilepsy, psychiatric disorders and pain. Here we report the cryo-electron microscopy structures of human Ca3.1 alone and in complex with a highly Ca3-selective blocker, Z944, at resolutions of 3.3~{\r A} and 3.1~{\r A}, respectively. The arch-shaped Z944 molecule reclines in the central cavity of the pore domain, with the wide end inserting into the fenestration on the interface between repeats II and III, and the narrow end hanging above the intracellular gate like a plug. The structures provide the framework for comparative investigation of the distinct channel properties of different Ca subfamilies.</p> },
  year = {2019},
  journal = {Nature},
  volume = {576},
  pages = {492-497},
  month = {2019 Dec},
  issn = {1476-4687},
  doi = {10.1038/s41586-019-1801-3},
  language = {eng},
}