@article{3675,
  keywords = {Animals, Mice, Embryo, Mammalian, Embryonic Development, Genome, CRISPR-Cas Systems, Homologous Recombination, Microinjections, Gene Editing, Gene Knock-In Techniques},
  author = {Bin Gu and Marina Gertsenstein and Eszter Posfai},
  title = {Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos.},
  abstract = { <p>Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.</p> },
  year = {2020},
  journal = {Methods Mol Biol},
  volume = {2066},
  pages = {89-100},
  month = {2020},
  issn = {1940-6029},
  doi = {10.1007/978-1-4939-9837-1_7},
  language = {eng},
}