@article{3403,
  keywords = {Repressor Proteins, Animals, Mice, Base Sequence, Transcription, Genetic, Humans, Computational Biology, Gene Library, Reproducibility of Results, Cell Cycle Proteins, Spindle Apparatus, RNA Interference, Calcium-Binding Proteins, Databases, Genetic, Genome, Cell Cycle Checkpoints, Mad2 Proteins},
  author = {Frederic Sigoillot and Susan Lyman and Jeremy Huckins and Britt Adamson and Eunah Chung and Brian Quattrochi and Randall King},
  title = {A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.},
  abstract = { <p>Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.</p> },
  year = {2012},
  journal = {Nat Methods},
  volume = {9},
  pages = {363-6},
  month = {2012 Feb 19},
  issn = {1548-7105},
  doi = {10.1038/nmeth.1898},
  language = {eng},
}