@article{3123,
  keywords = {Cryoelectron Microscopy, Carbon Dioxide, Organelle Biogenesis, Algal Proteins, Chlamydomonas reinhardtii, Chloroplasts, Ribulose-Bisphosphate Carboxylase},
  author = {Elizabeth Rosenzweig and Bin Xu and Luis Cuellar and Antonio Martinez-Sanchez and Miroslava Schaffer and Mike Strauss and Heather Cartwright and Pierre Ronceray and J{\"u}rgen Plitzko and Friedrich F{\"o}rster and Ned Wingreen and Benjamin Engel and Luke Mackinder and Martin Jonikas},
  title = {The Eukaryotic CO-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.},
  abstract = { <p>Approximately 30\%-40\% of global CO fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid.~Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.</p> },
  year = {2017},
  journal = {Cell},
  volume = {171},
  pages = {148-162.e19},
  month = {2017 Sep 21},
  issn = {1097-4172},
  doi = {10.1016/j.cell.2017.08.008},
  language = {eng},
}