@article{3100,
  keywords = {Gene Expression Regulation, Bacterial, Bacterial Proteins, Signal Transduction, Recombinant Fusion Proteins, Receptors, Cell Surface, Green Fluorescent Proteins, Escherichia coli Proteins, Luminescent Proteins, Adaptation, Physiological, Microfluidic Analytical Techniques, Temperature, Serine, Fluorescence Resonance Energy Transfer, Aspartic Acid, Chemotactic Factors, Escherichia coli K12, Methyl-Accepting Chemotaxis Proteins, Taxis Response},
  author = {Anja Paulick and Vladimir Jakovljevic and SiMing Zhang and Michael Erickstad and Alex Groisman and Yigal Meir and William Ryu and Ned Wingreen and Victor Sourjik},
  title = {Mechanism of bidirectional thermotaxis in .},
  abstract = { <p>In bacteria various tactic responses are mediated by the same cellular pathway, but sensing of physical stimuli remains poorly understood. Here, we combine an in-vivo analysis of the pathway activity with a microfluidic taxis assay and mathematical modeling to investigate the thermotactic response of . We show that in the absence of chemical attractants  exhibits a steady thermophilic response, the magnitude of which decreases at higher temperatures. Adaptation of wild-type cells to high levels of chemoattractants sensed by only one of the major chemoreceptors leads to inversion of the thermotactic response at intermediate temperatures and bidirectional cell accumulation in a thermal gradient. A mathematical model can explain this behavior based on the saturation-dependent kinetics of adaptive receptor methylation. Lastly, we find that the preferred accumulation temperature corresponds to optimal growth in the presence of the chemoattractant serine, pointing to a physiological relevance of the observed thermotactic behavior.</p> },
  year = {2017},
  journal = {Elife},
  volume = {6},
  month = {2017 Aug 03},
  issn = {2050-084X},
  doi = {10.7554/eLife.26607},
  language = {eng},
}