@article{2499,
  keywords = {Humans, In Vitro Techniques, Cells, Cultured, Adult, Cysteine, Fibroblasts, Cell Movement, Oxidation-Reduction, Middle Aged, Administration, Cutaneous, Esthetics, Follow-Up Studies, Patient Satisfaction, Prospective Studies, Skin Aging, Treatment Outcome},
  author = {Jos{\'e} Fern{\'a}ndez and Karl Rouzard and Michael Voronkov and Kristen Huber and Corey Webb and Jeffry Stock and Maxwell Stock and Joel Gordon and Eduardo P{\'e}rez},
  title = {In vitro and clinical evaluation of SIG1273: a cosmetic functional ingredient with a broad spectrum of anti-aging and antioxidant activities.},
  abstract = { <p><b>BACKGROUND: </b>Isoprenylcysteine (IPC) small molecules were identified as a new class of anti-inflammatory compounds over 20~years ago. Since then, they have been developed as novel cosmetic functional ingredients (CFI) and topical drug candidates. SIG1273 is a second generation CFI that has previously been shown to provide a broad spectrum of benefits for the skin through its anti-inflammatory and antimicrobial properties.</p><p><b>OBJECTIVE: </b>To determine whether SIG1273 possesses anti-aging properties in~vitro and evaluate the tolerability and activity of SIG1273 when applied topically to human subjects.</p><p><b>METHODS: </b>To model photoaging in~vitro, human dermal fibroblasts (HDFs) were exposed in culture to UVA to induce collagenase (MMP-1) production. An in~vitro wound-healing model was based on the activation of HDF migration into cell-free tissue culture surface. Hydrogen peroxide-induced oxidative stress was performed using HDFs to measure intracellular ROS activity. Radical scavenging capacity was determined using a colorimetric antioxidant assay kit (ABTS method). Lastly, a 4-week, 29-subject study was performed in which SIG1273 was applied topically as a cream to assess its tolerance and activity in reducing the appearance of aging.</p><p><b>RESULTS: </b>In vitro studies demonstrate SIG1273 inhibits UVA-induced MMP-1 production, hydrogen peroxide-induced oxidative stress and promotes wound healing. Moreover, SIG1273 was shown to be a radical scavenging antioxidant. Clinical assessment of SIG1273 cream (0.25\%) showed it was well tolerated with significant improvement in the appearance of fine lines, coarse wrinkles, radiance/luminosity, pore size, texture/smoothness, hydration and increased firmness.</p><p><b>CONCLUSIONS: </b>SIG1273 represents a novel CFI with antioxidant, anti-aging, and anti-inflammatory properties that when applied topically is well tolerated and provides benefits to individuals with aging skin.</p> },
  year = {2016},
  journal = {J Cosmet Dermatol},
  volume = {15},
  pages = {150-7},
  month = {2016 Jun},
  issn = {1473-2165},
  doi = {10.1111/jocd.12206},
  language = {eng},
}