Instruments

	Leica sp5 5 channel spectral imaging system with excitations at 405, 458, 476, 488, 514, 561, 594 and 633. Spectral seperation of overlapping fluorophores Imaging sppeds from 10 to 1,400 hertz with mirror scanning Resonant scanning at 8,000 hertz [25 frames / second at 512 x 512 pixel resolution]. Fast z- stepping with galvo z stage Live cell imaging at controlled temperatures employing warner heating unit Frap, fret , flip and visible uncaging
	Perkinelmer spinning disk confocal This confocal microscope was installed in August 2005. We recently upgraded to 3 solid state lasers, a 50mWatt 488, a 50mWatt 560 and a 40 mWatt 640 providing 3 channel imaging plus transmitted light. We also just added a 100 mWatt 285 Micropoint laser system that can produce a 365 nm line for ablation, 405 nm line for photo-activation or photo-conversion and a 4354 nm line for photo-bleaching to perform FRAP experiments. System is located in Moffett 121.
	Prairie ultima multi-photon system 2 channel imaging using 2 GaAsp detectors per channel. A Coherent Chameleon Ultima II pulse laser, tunable from 680 to 1080 nms. Dual scanning system - galvo scanners and an AOD scanner with scan speeds up to 25 fps at 512 x 512 resolution Fast Prior piezo z-stage with 500 microns of travel 405 laser for ablation, photo-activation and photo-conversion Environmental chamber for live cell mammalian imaging. Temp. Range 25 to 45 degrees C.\ Objective lenses: 1.0 NA20 x and 40 x Plan Apo's, 1.3 NA 40 x Plan Apo 0.2 NA 5X Plan fluor and 10 x 0.6 NA Plan Apo
	Zeiss lsm 510 confocal system The Zeiss LSM 510 confocal system was installed in October 1997. The system has a 3rd generation scanhead with CFP and YFP dichroics and filter sets added. The 510 has 4 lasers producing 6 lines [364, 458, 488, 514, 543 and 633 nms.] for fluorochrome excitation, 4 color detectors and a transmitted light detector. The 510's capabilities: Simultaneous imaging 4 channels with combined merged image view UV imaging into live specimens with C-Apo water immersion objectives Imaging of CFP / GFP / YFP in live specimens for time course studies at ambient and incubator temperatures and CO2 YFP and CFP imaging in fixed and live specimens Sequential and bi-directional scanning to avoid channel cross talk Image resolution from 256 x 256 up to 2048 x 2048 pixels Image processing in 3d, stereo, or 2 1/2 d for histogram over area UV laser ablation - FRAP studies - FRET - cage release of cpds. & fluorochromes