Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

TitleSingle-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.
Publication TypeJournal Article
Year of Publication2017
AuthorsDouam, F, Hrebikova, G, Albrecht, YESoto, Sellau, J, Sharon, Y, Ding, Q, Ploss, A
JournalNat Commun
Volume8
Pagination14781
Date Published2017 Mar 14
ISSN2041-1723
Abstract

Positive-sense RNA viruses pose increasing health and economic concerns worldwide. Our limited understanding of how these viruses interact with their host and how these processes lead to virulence and disease seriously hampers the development of anti-viral strategies. Here, we demonstrate the tracking of (+) and (-) sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models. Our results provide insights into how a prototypic flavivirus, yellow fever virus (YFV-17D), differentially interacts with murine and human hematopoietic cells in these mouse models and how these dynamics influence distinct outcomes of infection. We detect (-) YFV-17D RNA in specific secondary lymphoid compartments and cell subsets not previously recognized as permissive for YFV replication, and we highlight potential virus-host interaction events that could be pivotal in regulating flavivirus virulence and attenuation.

DOI10.1038/ncomms14781
Alternate JournalNat Commun
PubMed ID28290449