Signal production and detection specificity in Vibrio CqsA/CqsS quorum-sensing systems.

TitleSignal production and detection specificity in Vibrio CqsA/CqsS quorum-sensing systems.
Publication TypeJournal Article
Year of Publication2011
AuthorsNg, W-L, Perez, LJ, Wei, Y, Kraml, C, Semmelhack, MF, Bassler, BL
JournalMol Microbiol
Volume79
Issue6
Pagination1407-17
Date Published2011 Mar
ISSN1365-2958
KeywordsBacterial Proteins, Gene Expression Regulation, Bacterial, Ketones, Protein Kinases, Quorum Sensing, Signal Transduction, Species Specificity, Vibrio, Vibrio cholerae
Abstract

<p>Quorum sensing is a process of bacterial cell-cell communication that enables populations of cells to carry out behaviours in unison. Quorum sensing involves detection of the density-dependent accumulation of extracellular signal molecules called autoinducers that elicit population-wide changes in gene expression. In Vibrio species, CqsS is a membrane-bound histidine kinase that acts as the receptor for the CAI-1 autoinducer which is produced by the CqsA synthase. In Vibrio cholerae, CAI-1 is (S)-3-hydroxytridecan-4-one. The C170 residue of V. cholerae CqsS specifies a preference for a ligand with a 10-carbon tail length. However, a phenylalanine is present at this position in Vibrio harveyi CqsS and other homologues, suggesting that a shorter CAI-1-like molecule functions as the signal. To investigate this, we purified the V. harveyi CqsS ligand, and determined that it is (Z)-3-aminoundec-2-en-4-one (Ea-C8-CAI-1) carrying an 8-carbon tail. The V. harveyi CqsA/CqsS system is exquisitely selective for production and detection of this ligand, while the V. cholerae CqsA/CqsS counterparts show relaxed specificity in both production and detection. We isolated CqsS mutants in each species that display reversed specificity for ligands. Our analysis provides insight into how fidelity is maintained in signal transduction systems.</p>

DOI10.1111/j.1365-2958.2011.07548.x
Alternate JournalMol. Microbiol.
PubMed ID21219472
PubMed Central IDPMC3285556
Grant List5R01AI054442 / AI / NIAID NIH HHS / United States
5R01GM065859 / GM / NIGMS NIH HHS / United States
GM082061 / GM / NIGMS NIH HHS / United States
R01 AI054442 / AI / NIAID NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859-10 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
/ / Howard Hughes Medical Institute / United States