RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis.

TitleRodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis.
Publication TypeJournal Article
Year of Publication2015
AuthorsMorgenstein, RM, Bratton, BP, Nguyen, JP, Ouzounov, N, Shaevitz, JW, Gitai, Z
JournalProc Natl Acad Sci U S A
Volume112
Issue40
Pagination12510-5
Date Published2015 Oct 6
ISSN1091-6490
KeywordsCell Wall, Cytoskeletal Proteins, Escherichia coli, Escherichia coli Proteins, Luminescent Proteins, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Mutation, Protein Binding, Rotation, Time-Lapse Imaging
Abstract

<p>The rod shape of most bacteria requires the actin homolog, MreB. Whereas MreB was initially thought to statically define rod shape, recent studies found that MreB dynamically rotates around the cell circumference dependent on cell wall synthesis. However, the mechanism by which cytoplasmic MreB is linked to extracytoplasmic cell wall synthesis and the function of this linkage for morphogenesis has remained unclear. Here we demonstrate that the transmembrane protein RodZ mediates MreB rotation by directly or indirectly coupling MreB to cell wall synthesis enzymes. Furthermore, we map the RodZ domains that link MreB to cell wall synthesis and identify mreB mutants that suppress the shape defect of ΔrodZ without restoring rotation, uncoupling rotation from rod-like growth. Surprisingly, MreB rotation is dispensable for rod-like shape determination under standard laboratory conditions but is required for the robustness of rod shape and growth under conditions of cell wall stress.</p>

DOI10.1073/pnas.1509610112
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID26396257
PubMed Central IDPMC4603514
Grant List1R01GM107384 / GM / NIGMS NIH HHS / United States
F32 GM103290 / GM / NIGMS NIH HHS / United States
F32 GM103290-01A1 / GM / NIGMS NIH HHS / United States
P50 GM071508 / GM / NIGMS NIH HHS / United States
R01 GM107384 / GM / NIGMS NIH HHS / United States