|Title||RNA surveillance via nonsense-mediated mRNA decay is crucial for longevity in daf-2/insulin/IGF-1 mutant C. elegans.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Son, HG, Seo, M, Ham, S, Hwang, W, Lee, D, An, SWoo A, Artan, M, Seo, K, Kaletsky, R, Arey, RN, Ryu, Y, Ha, CMan, Kim, YKi, Murphy, CT, Roh, T-Y, Nam, HGil, Lee, S-JV|
|Date Published||2017 Mar 09|
Long-lived organisms often feature more stringent protein and DNA quality control. However, whether RNA quality control mechanisms, such as nonsense-mediated mRNA decay (NMD), which degrades both abnormal as well as some normal transcripts, have a role in organismal aging remains unexplored. Here we show that NMD mediates longevity in C. elegans strains with mutations in daf-2/insulin/insulin-like growth factor 1 receptor. We find that daf-2 mutants display enhanced NMD activity and reduced levels of potentially aberrant transcripts. NMD components, including smg-2/UPF1, are required to achieve the longevity of several long-lived mutants, including daf-2 mutant worms. NMD in the nervous system of the animals is particularly important for RNA quality control to promote longevity. Furthermore, we find that downregulation of yars-2/tyrosyl-tRNA synthetase, an NMD target transcript, by daf-2 mutations contributes to longevity. We propose that NMD-mediated RNA surveillance is a crucial quality control process that contributes to longevity conferred by daf-2 mutations.
|Alternate Journal||Nat Commun|