Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion.

TitleRemodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion.
Publication TypeJournal Article
Year of Publication2015
AuthorsBosse, JB, Hogue, IB, Feric, M, Thiberge, SY, Sodeik, B, Brangwynne, CP, Enquist, LW
JournalProc Natl Acad Sci U S A
Volume112
Issue42
PaginationE5725-33
Date Published2015 Oct 20
ISSN1091-6490
KeywordsCapsid, Cell Line, Cell Nucleus, Diffusion, Herpesviridae, Microscopy, Fluorescence, Protein Transport, Virus Replication
Abstract

<p>The nuclear chromatin structure confines the movement of large macromolecular complexes to interchromatin corrals. Herpesvirus capsids of approximately 125 nm assemble in the nucleoplasm and must reach the nuclear membranes for egress. Previous studies concluded that nuclear herpesvirus capsid motility is active, directed, and based on nuclear filamentous actin, suggesting that large nuclear complexes need metabolic energy to escape nuclear entrapment. However, this hypothesis has recently been challenged. Commonly used microscopy techniques do not allow the imaging of rapid nuclear particle motility with sufficient spatiotemporal resolution. Here, we use a rotating, oblique light sheet, which we dubbed a ring-sheet, to image and track viral capsids with high temporal and spatial resolution. We do not find any evidence for directed transport. Instead, infection with different herpesviruses induced an enlargement of interchromatin domains and allowed particles to diffuse unrestricted over longer distances, thereby facilitating nuclear egress for a larger fraction of capsids.</p>

DOI10.1073/pnas.1513876112
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID26438852
PubMed Central IDPMC4620878
Grant ListNS033506 / NS / NINDS NIH HHS / United States
DP2 GM105437 / GM / NIGMS NIH HHS / United States
NS060699 / NS / NINDS NIH HHS / United States
R01 NS033506 / NS / NINDS NIH HHS / United States
R01 NS060699 / NS / NINDS NIH HHS / United States
R37 NS033506 / NS / NINDS NIH HHS / United States