Quorum-sensing non-coding small RNAs use unique pairing regions to differentially control mRNA targets.

TitleQuorum-sensing non-coding small RNAs use unique pairing regions to differentially control mRNA targets.
Publication TypeJournal Article
Year of Publication2012
AuthorsShao, Y, Bassler, BL
JournalMol Microbiol
Volume83
Issue3
Pagination599-611
Date Published2012 Feb
ISSN1365-2958
KeywordsBacterial Proteins, Base Pairing, Base Sequence, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Quorum Sensing, RNA Processing, Post-Transcriptional, RNA, Bacterial, RNA, Messenger, RNA, Small Untranslated, Trans-Activators, Vibrio
Abstract

<p>Quorum sensing is a mechanism of cell-cell communication that bacteria use to control collective behaviours including bioluminescence, biofilm formation and virulence factor production. In the Vibrio harveyi and Vibrio cholerae quorum-sensing circuits, multiple non-coding small regulatory RNAs called the quorum-regulated small RNAs (Qrr sRNAs) function to establish the global quorum-sensing gene expression pattern by modulating translation of multiple mRNAs encoding quorum-sensing regulatory factors. Here we show that the Qrr sRNAs post-transcriptionally activate production of the low cell density master regulator AphA through base pairing to aphA mRNA, and this is crucial for the accumulation of appropriate levels of AphA protein at low cell density. We find that the Qrr sRNAs use unique pairing regions to discriminate between their different targets. Qrr1 is not as effective as Qrr2-5 in activating aphA because Qrr1 lacks one of two required pairing regions. However, Qrr1 is equally effective as the other Qrr sRNAs at controlling targets like luxR and luxO because it harbours all of the required pairing regions for these targets. Sequence comparisons reveal that Vibrionaceae species possessing only qrr1 do not have the aphA gene under Qrr sRNA control. Our findings suggest co-evolving relationships between particular Qrr sRNAs and particular mRNA targets.</p>

DOI10.1111/j.1365-2958.2011.07959.x
Alternate JournalMol. Microbiol.
PubMed ID22229925
PubMed Central IDPMC3262071
Grant List5R01AI054442 / AI / NIAID NIH HHS / United States
5R01GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859-09 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
/ / Howard Hughes Medical Institute / United States