Quantifying the integration of quorum-sensing signals with single-cell resolution.

TitleQuantifying the integration of quorum-sensing signals with single-cell resolution.
Publication TypeJournal Article
Year of Publication2009
AuthorsLong, T, Tu, KC, Wang, Y, Mehta, P, Ong, NP, Bassler, BL, Wingreen, NS
JournalPLoS Biol
Volume7
Issue3
Paginatione68
Date Published2009 Mar 24
ISSN1545-7885
Keywords4-Butyrolactone, Bacterial Proteins, Gene Expression Regulation, Bacterial, Homoserine, Lactones, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Quorum Sensing, Signal Transduction, Vibrio
Abstract

<p>Cell-to-cell communication in bacteria is a process known as quorum sensing that relies on the production, detection, and response to the extracellular accumulation of signaling molecules called autoinducers. Often, bacteria use multiple autoinducers to obtain information about the vicinal cell density. However, how cells integrate and interpret the information contained within multiple autoinducers remains a mystery. Using single-cell fluorescence microscopy, we quantified the signaling responses to and analyzed the integration of multiple autoinducers by the model quorum-sensing bacterium Vibrio harveyi. Our results revealed that signals from two distinct autoinducers, AI-1 and AI-2, are combined strictly additively in a shared phosphorelay pathway, with each autoinducer contributing nearly equally to the total response. We found a coherent response across the population with little cell-to-cell variation, indicating that the entire population of cells can reliably distinguish several distinct conditions of external autoinducer concentration. We speculate that the use of multiple autoinducers allows a growing population of cells to synchronize gene expression during a series of distinct developmental stages.</p>

DOI10.1371/journal.pbio.1000068
Alternate JournalPLoS Biol.
PubMed ID19320539
PubMed Central IDPMC2661960
Grant List5 R01 AI054442 / AI / NIAID NIH HHS / United States
5 R01 GM065859 / GM / NIGMS NIH HHS / United States
K25 GM086909 / GM / NIGMS NIH HHS / United States
K25 GM086909-01 / GM / NIGMS NIH HHS / United States
P50 GM071508 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States