A qrr noncoding RNA deploys four different regulatory mechanisms to optimize quorum-sensing dynamics.

TitleA qrr noncoding RNA deploys four different regulatory mechanisms to optimize quorum-sensing dynamics.
Publication TypeJournal Article
Year of Publication2015
AuthorsFeng, L, Rutherford, ST, Papenfort, K, Bagert, JD, van Kessel, JC, Tirrell, DA, Wingreen, NS, Bassler, BL
JournalCell
Volume160
Issue1-2
Pagination228-40
Date Published2015 Jan 15
ISSN1097-4172
KeywordsBase Sequence, Escherichia coli, Inverted Repeat Sequences, Molecular Sequence Data, Nucleic Acid Conformation, Quorum Sensing, RNA, Bacterial, RNA, Messenger, RNA, Small Untranslated, Vibrio
Abstract

<p>Quorum sensing is a cell-cell communication process that bacteria use to transition between individual and social lifestyles. In vibrios, homologous small RNAs called the Qrr sRNAs function at the center of quorum-sensing pathways. The Qrr sRNAs regulate multiple mRNA targets including those encoding the quorum-sensing regulatory components luxR, luxO, luxM, and aphA. We show that a representative Qrr, Qrr3, uses four distinct mechanisms to control its particular targets: the Qrr3 sRNA represses luxR through catalytic degradation, represses luxM through coupled degradation, represses luxO through sequestration, and activates aphA by revealing the ribosome binding site while the sRNA itself is degraded. Qrr3 forms different base-pairing interactions with each mRNA target, and the particular pairing strategy determines which regulatory mechanism occurs. Combined mathematical modeling and experiments show that the specific Qrr regulatory mechanism employed governs the potency, dynamics, and competition of target mRNA regulation, which in turn, defines the overall quorum-sensing response.</p>

DOI10.1016/j.cell.2014.11.051
Alternate JournalCell
PubMed ID25579683
PubMed Central IDPMC4313533
Grant List5R01GM065859 / GM / NIGMS NIH HHS / United States
F32 AI085922 / AI / NIAID NIH HHS / United States
F32AI085922 / AI / NIAID NIH HHS / United States
R01 GM062523 / GM / NIGMS NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
R01 GM082938 / GM / NIGMS NIH HHS / United States
R01GM062523 / GM / NIGMS NIH HHS / United States
R01GM082938 / GM / NIGMS NIH HHS / United States