Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles.

TitlePlanar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles.
Publication TypeJournal Article
Year of Publication2008
AuthorsDevenport, D, Fuchs, E
JournalNat Cell Biol
Volume10
Issue11
Pagination1257-68
Date Published2008 Nov
ISSN1476-4679
KeywordsAnimals, Body Patterning, Cell Polarity, Cells, Cultured, Embryo, Mammalian, Epidermis, Frizzled Receptors, Gene Expression, Hair, Hair Follicle, Immunohistochemistry, Keratinocytes, Mice, Mice, Knockout, Mice, Mutant Strains, Morphogenesis, Mutation, Nerve Tissue Proteins, Receptors, G-Protein-Coupled, Transfection
Abstract

<p>Mammalian body hairs align along the anterior-posterior (A-P) axis and offer a striking but poorly understood example of global cell polarization, a phenomenon known as planar cell polarity (PCP). We have discovered that during embryogenesis, marked changes in cell shape and cytoskeletal polarization occur as nascent hair follicles become anteriorly angled, morphologically polarized and molecularly compartmentalized along the A-P axis. Hair follicle initiation coincides with asymmetric redistribution of Vangl2, Celsr1 and Fzd6 within the embryonic epidermal basal layer. Moreover, loss-of-function mutations in Vangl2 and Celsr1 show that they have an essential role in hair follicle polarization and orientation, which develop in part through non-autonomous mechanisms. Vangl2 and Celsr1 are both required for their planar localization in vivo, and physically associate in a complex in vitro. Finally, we provide in vitro evidence that homotypic intracellular interactions of Celsr1 are required to recruit Vangl2 and Fzd6 to sites of cell-cell contact.</p>

DOI10.1038/ncb1784
Alternate JournalNat. Cell Biol.
PubMed ID18849982
PubMed Central IDPMC2607065
Grant ListF32 AR053019 / AR / NIAMS NIH HHS / United States
R01 AR027883-29 / / None / None
R01 AR031737-26 / AR / NIAMS NIH HHS / United States
R01 AR031737 / AR / NIAMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
R01 AR031737-26 / / None / None
R01 AR027883-29 / AR / NIAMS NIH HHS / United States
R01 AR027883 / AR / NIAMS NIH HHS / United States
R01 AR27883 / AR / NIAMS NIH HHS / United States